For research use only.
| Verified Samples |
Verified Samples in WB: K562, Mouse brain Verified Samples in IHC: Human liver cancer |
| Dilution | WB 1:500-1:2000, IHC 1:40-1:200 |
| Clonality | Polyclonal |
| Immunogen | Synthetic peptide of human VAMP4 |
| Abbre | VAMP4 |
| Synonyms | VAMP-4, VAMP24., VAMP4/TAF3 Fusion, Vesicle Associated Membrane Protein 4, Vesicle-Associated Membrane Protein 4 |
| Swissprot | |
| Calculated MW | 16 kDa |
| Observed MW |
Refer to figures
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Endosome, Golgi apparatus, Golgi apparatus, Golgi membrane, trans-Golgi network membrane, Lysosome, Plasma Membrane, Other locations: cell surface, clathrin-coated vesicle membrane, integral component of synaptic vesicle membrane, SNARE complex, synaptic vesicle, transport vesicle. |
| Concentration | 0.7 mg/mL |
| Buffer | PBS with 0.05% NaN3 and 40% Glycerol,pH7.4 |
| Purification Method | Antigen affinity purification |
| Research Areas | Signal Transduction |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane.The protein encoded by this gene is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family.This protein may play a role in trans-Golgi network-to-endosome transport. |
Other Clones
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