VD(Vitamin D) ELISA Kit (E-EL-0012)

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal VD. During the reaction, Universal VD in the sample or standard competes with a fixed amount of Universal VD on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal VD. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal VD in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Vitamin D is a fat-soluble vitamin made mainly from 7-dehydrocholesterol in the human body and ergosterol in plants or yeast by ultraviolet irradiation. Like vitamins A, E, and K, it is a fat-soluble vitamin that dissolves in fat and is best absorbed when taken at the same time as some fats. Vitamin D deficiency is common around the world and causes the body to absorb less calcium and phosphate to maintain healthy bones. The way the body supplements vitamin D is mainly through sun exposure and diet. Sun exposure can ensure the synthesis of vitamin D within the body, which can significantly treat the improvement of calcium deficiency caused by insufficient vitamin D. Therefore, as for the "vitamin D" mentioned in the question, according to the information I have searched, it can be concluded that vitamin D is a fat-soluble vitamin that is essential for human health, and its excessive intake will accumulate in fat tissue and produce toxicity, and the average adult is not deficient in vitamin D due to frequent exposure to sunlight. At the same time, vitamin D deficiency is common worldwide, and its deficiency can lead to reduced absorption of calcium and phosphate, affecting bone health. In addition, the main way the body supplements vitamin D is through sun exposure and diet.