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For research use only.

Verified Samples Verified Samples in WB: Hela, HepG2, SW480, B16, PC12, Mouse lung
Verified Samples in IHC: Human lung cancer, Human colon, Human liver cancer, Human kidney
Dilution WB 1:500-1:2000,  IHC 1:200-1:800
Isotype IgG
Host Rabbit
Reactivity Human,  Mouse,  Rat
Applications WB,  IHC
Clonality Polyclonal
Immunogen KLH conjugated Synthetic peptide corresponding to Mouse vimentin
Abbre Vimentin
Synonyms CTRCT30,  Epididymis luminal protein 113,  FLJ36605,  HEL113,  VIM,  VIME,  Vimentin
Swissprot
Calculated MW 57 kDa
Observed MW 57 kDa

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Cellular Localization Cytoplasm
Concentration 1.1 mg/mL
Buffer PBS with 0.02% sodium azide, 1% protective protein and 50% glycerol, pH7.4
Purification Method Affinity purification
Research Areas Cancer,  Developmental Biology,  Neuroscience,  Signal Transduction,  Stem Cells
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended.
background Vimentin,also named as VIM,belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells,especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10 (124092),suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein,55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level.
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Unconjugated

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