Xanthine/Hypoxanthine Fluorometric Assay Kit (E-BC-F024)
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For research use only.
| Detection Principle |
Xanthine and hypoxanthine are important alkaloid purines that can be used to treat symptoms such as asthma relief and bronchodilation. Hypoxanthine can be oxidized by xanthine oxidase to form xanthine. Xanthine is catalyzed by xanthine oxidase to form uric acid. When xanthine oxidase is deficient, xanthine accumulates in urine and blood, inducing hyperuricemia and gout, and eventually leading to renal failure. The detection principle of this kit: Xanthine/hypoxanthine generates intermediate products under the catalysis of enzymes. Under the action of other enzymes, the intermediate products react with the probe to form fluorescent substances. By substituting the fluorescence values into the standard curve, the total content of xanthine and hypoxanthine in the sample can be calculated. |
| Sample Type | Serum,Plasma,Animal tissue,Cell |
| Detection Method | Fluorometric method |
| Detection Instrument | Fluorescence microplate reader (Ex/Em=535 nm/587 nm) |
| Research Area | Oxidative Stress |
| Other Reagents Required | Normal saline (0.9% NaCl) |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.10 µmol/L |
| Detection Range | 0.10-50 µmol/L |
| Precision | inter-assay CV: 2.9-8.3% | intra-assay CV: 0.4-3.2% |
| Sample Volume | 100-150 μL |
| Assay Time | 1 h 50 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 2-20 |
| Rat serum | 1 |
| 10% Rat kidney tissue homogenate | 5-20 |
| 10% Rat brain tissue homogenate | 2-10 |
| 10% Rat lung tissue homogenate | 1 |
| 10% Rat liver tissue homogenate | 5-10 |
| 10% Rat heart tissue homogenate | 8-20 |
| 10% Rat spleen tissue homogenate | 8-20 |
| 1×10^6 HL-60 cells | 1 |
| 1×10^6 293T cells | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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