YY1 Monoclonal Antibody (AN005360L)
For research use only.
| Verified Samples | Verified Samples in WB: HeLa, MCF-7, Molt-4, C2C12, PC-12 |
| Dilution | WB 1:1000-1:2000 |
| Isotype | IgG2b |
| Host | Mouse |
| Reactivity | Human, Mouse, Rat |
| Applications | WB |
| Clonality | Monoclonal |
| Immunogen | Recombinant human YY1 protein expressed by E.coli |
| Abbre | YY1 |
| Synonyms | YY1, Transcriptional repressor protein YY1, Delta transcription factor, INO80 complex subunit S, NF-E1, Yin and yang 1 (YY-1), INO80S |
| Swissprot | |
| Calculated MW | 45 kDa |
| Observed MW |
65 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Nucleus matrix |
| Concentration | 1 mg/mL |
| Buffer | PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4 |
| Purification Method | Protein A/G Purification |
| Research Areas | Epigenetics and Nuclear Signaling, Neuroscience, Isotype, Loading Controls |
| Clone No. | 4C7 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended. |
| background | YY1 is a widely distributed transcription factor belonging to the GLI-Kruppel class of zinc finger proteins. This protein is involved in inhibiting and activating a variety of promoters. YY1 can directly introduce histone deacetylase and histone acetyltransferase into the promoter, thereby activating or inhibiting the promoter, so that histone modification can act on YY1. Multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. Binds to the consensus sequence 5'-CCGCCATNTT-3'; some genes have been shown to contain a longer binding motif allowing enhanced binding; the initial CG dinucleotide can be methylated greatly reducing the binding affinity. The effect on transcription regulation is depending upon the context in which it binds and diverse mechanisms of action include direct activation or repression, indirect activation or repression via cofactor recruitment, or activation or repression by disruption of binding sites or conformational DNA changes. Its activity is regulated by transcription factors and cytoplasmic proteins that have been shown to abrogate or completely inhibit YY1-mediated activation or repression. |
Other Clones
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Unconjugated
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