For research use only.
| Verified Samples |
Verified Samples in WB: PC-3, Hela Verified Samples in IHC: Human esophagus cancer, Human liver cancer |
| Dilution | WB 1:500-1:2000, IHC 1:50-1:300 |
| Clonality | Polyclonal |
| Immunogen | Fusion protein of human ZFYVE27 |
| Abbre | ZFYVE27 |
| Synonyms | Protrudin, RP11 459F3.2, SPG33 , ZFY27, ZFYVE27, Zinc finger FYVE domain containing protein 27 , Zinc finger FYVE domain-containing protein 27, zinc finger FYVE domain containing 27 |
| Swissprot | |
| Calculated MW | 46 kDa |
| Observed MW |
Refer to figures
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Recycling endosome membrane. Endoplasmic reticulum membrane. Cell projection>growth cone membrane. Cell membrane. Localizes at both dendrites and axons. |
| Concentration | 0.9 mg/mL |
| Buffer | PBS with 0.05% NaN3 and 40% Glycerol,pH7.4 |
| Purification Method | Antigen affinity purification |
| Research Areas | Neuroscience |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | This gene encodes a protein with several transmembrane domains, a Rab11-binding domain and a lipid-binding FYVE finger domain. The encoded protein appears to promote neurite formation. A mutation in this gene has been reported to be associated with hereditary spastic paraplegia, however the pathogenicity of the mutation, which may simply represent a polymorphism, is unclear. |
Other Clones
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