ELISA Research
ELISA Research FAQs
In order to provide better customer support, we have published Answers to the most Frequently Asked Questions (FAQs) about Elabscience® products.
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It is recommended to use PBS, RIPA has an impact on the detection of some indicators, try to avoid using it. Depending on whether the protein you want to study is on the membrane, in the nucleus or in the cytoplasm, RIPA lysate is less efficient for extracting proteins on the membrane and in the nucleus. If that's the case, buy a specialized cell structure protein extraction kit. When ELISA detects cytokines, it is necessary to select the corresponding lysate to treat the sample according to the different cytokines detected.
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TGF-β1 is usually present in an inactive form in biological samples and must be activated before TGF-β1 activity can be detected. The detection of serum, plasma, cell supernatant and other secretory samples requires activation treatment. Intracellular samples such as tissue homogenate supernatant can be detected without activation.
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It can be used, but cell culture requires a sterile environment, ELISA does not need to be sterile, we recommend that customers try not to use this instrument, to avoid doing other experiments such as cell culture caused by pollution, you can choose a conventional oven or water bath
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According to the different bottom, it is divided into flat bottom, U-shaped bottom, V-shaped bottom and so on. The index of refraction of the flat bottom is low, which is suitable for detection in the enzyme reader. According to the color can be divided into transparent, black, white. Transparent is commonly used for the most general enzyme-linked immunoassay.
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E-EL-E605 SARS-CoV-2 Spike Protein S1 RBD ELISA Kit, Immunogenic sequence (AA: 319-541 Accession: P0DTC2), the S1 RBD protein identified by our kit is partially inconsistent with the customer's recombinant protein S1 415-636, and due to the difference in spatial conformation of the recombinant protein, the customer's protein is not sure to be recognized.
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We haven't verified human hair samples internally, so we recommend a preliminary test. Treatment method :ELISA was used to determine hair cortisol. The extraction process involves successive incubation in methanol and acetone, repeated twice
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The sample does not need to dilute into a uniform concentration and then the sample is loaded. The tissue sample can be made into 10% tissue homogenate, and then the total concentration is detected by BCA method, and then the ELISA test is performed. The concentration of target protein detected by ELISA/the concentration of total tissue protein can be obtained in the tissue sample (pg/mg prot).
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When cracking MCM7 cells, customers can directly pre-cool 0.01M PBS and trypsin according to the instructions, but pay attention to ensure the cell growth state and ensure that the number of cells reaches 10^6
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It will have an impact. Because its aqueous solution can denature the protein, it will affect the HRP activity and interfere with the overall detection, so it is not suitable for ELISA method. The addition of sodium azide is not recommended, but customers should take care to avoid contamination during urine collection.
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E-EL-0009 FA/VB9 is a universal ELISA kit that mainly detects folic acid content in biological liquids of all species (mainly human, rat and mouse). We have not verified folic acid in feed internally, but at present, the content of folic acid in feed is mostly detected by microbial detection methods
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E-UNEL-H0163 Uncoated Human IL-27(Interleukin 27) ELISA Kit IL-27, Both p28 and EBI3 subunits can be detected.
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The antigen is diluted with coated solution, the first antibody is diluted with universal sample diluent or ready-to-use biotinized antibody diluent, and the second antibody is diluted with ready-to-use HRP enzyme conjugate.
