ELISA Research
Why do we recommend pre-testing? How to conduct a pre-experiment?
Source: Elabscience®Published: Mar 05,2024
The purpose of the pre-test is: ① to determine whether the kit is suitable for your sample detection, so as to avoid the waste of kits and samples; ② To help you get familiar with the operating process of the kit, especially the customers who use the ELISA kit for the first time/change the experimental brand; ③ Determine whether the sample is diluted or not and whether the dilution ratio is suitable for the determination of this kit and confirm the optimal sample dilution information; ④ Help to understand the experimental phenomena or problems that may occur during the experiment, so as to make timely adjustments;
Specific methods: Before the formal test, 2-3 samples with large differences are selected to dilute the samples with different concentrations, and then pre-test is conducted according to the experimental procedures in the instructions. According to the results of the pre-test, the optimal dilution ratio of the samples is determined combined with the detection range of the kit.