ELISA Research
How can I tell if my sample needs to be diluted or processed?
Source: Elabscience®Published: Mar 05,2024
The detection range of the kit is not the same as the concentration range of the substance to be measured in the sample. It is recommended to estimate the concentration of the substance to be measured in the sample through relevant literature before the experiment and determine the actual concentration of the sample through pre-experiment. If it is a routine sample type, you can consult ELISA technical support to obtain the recommended dilution of the sample and schedule pre-laboratory testing.
If the concentration of the substance to be measured in the sample is too high or too low, dilute or concentrate the sample appropriately. If the concentration of analyte in the sample is much lower than the minimum detection line of the kit, it is recommended to choose a kit with higher sensitivity for detection.