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Protein Purification & Separation
Source: Elabscience®Published: Feb 05,2024
For most researchers in biochemistry, structural biology, and functional proteomics, a large amount of time and energy is devoted to the separation and purification of specific proteins or other biomolecules. This step is one of the most basic and important procedures in experimentation, and it is crucial for the accuracy of laboratory results and the management of experimental costs. Only by performing this process efficiently can we remain competitive with other researchers.
So, who is the 'clutch player' in this 'nonexplosive warfare'? The answer is high-quality chromatographic media. Elabscience® offers an extensive range of chromatographic media for the separation and purification of most biomolecules. We have curated our best products for all types of packing to help you separate and purify your target molecules easily and efficiently. All of these products have been rigorously verified by our skilled technicians, ensuring they are high-quality and can help you reduce costs and achieve favorable results. View our offerings and see how Elabscience® can assist with your biochemical research.
Gel filtration chromatography
Gel filtration chromatography, also known as size exclusion chromatography (SEC), is a chromatographic method that separates molecules based on their size or molecular weight. SEC is used for a wide range of separations and purifications of components with large molecular weight differences and low resolution requirements, such as proteins, polysaccharides, and other macromolecules.
Cat. No. | Product Name | Matrix | Particle Size Range | Average Particle Size |
E-CM-GF01 | Focurose 4B | 4% agarose | 45-165 µm | 90 µm |
E-CM-GF02 | Focurose 6B | 6% agarose | 45-165 µm | 90 µm |
E-CM-GF06 | Focurose CL-6B | Lowly cross-linked 6% agarose | 45-165 µm | 90 µm |
E-CM-GF07 | Focurose 4FF | Highly cross-linked 4% agarose | 45-165 µm | 90 µm |
E-CM-GF08 | Focurose 6FF | Highly cross-linked 6% agarose | 45-165 µm | 90 µm |
Affinity chromatography
Affinity chromatography is a powerful technique for separating and purifying proteins based on the highly specific interactions between an antigen and antibody, enzyme and substrate, or receptor and ligand. These interactions include hydrogen bonding, ionic interactions, disulfide bridges, and hydrophobic interactions. Affinity chromatography is widely recognized for its high selectivity, high resolution, and high capacity.
Cat. No. | Product Name | Matrix | Binding Capacity |
E-CM-AF03 | NHS Focurose 4FF | Highly cross-linked 4% agarose | 16-23 μmol /mL (media) |
E-CM-AF04 | GST Focurose 4FF | Highly cross-linked 4% agarose | 15-25mg (GST-tagged protein)/mL (medium) |
E-CM-AF06 | Ni Focurose 6FF (TED) | Highly cross-linked 6% agarose | 20-30 mg (His-tagged protein)/mL (medium) |
E-CM-AF07 | Ni Focurose 6FF (IMAC) | Highly cross-linked 6% agarose | 45 mg (His-tagged protein)/mL (medium) |
E-CM-AF08 | Ni Focurose 6FF (IDA) | Highly cross-linked 4% agarose | 40-50 mg (His-tagged protein)/mL (medium) |
E-CM-AF13 | Benzamidine Focurose 4FF(HS) | Highly cross-linked 4% agarose | 25-50 mg (Trypsase)/mL (media) |
E-CM-AF17 | CNBr Focurose 4FF | Highly cross-linked 4% agarose | 30 mg(Trypsinogen)/mL(media) |
E-CM-AF18 | Epoxy Focurose 4FF | Highly cross-linked 4% agarose | 25 μmol /mL(media) |
Ion exchange chromatography
Ion exchange chromatography (IEX) is a practical chromatography technique that separates ions and polar molecules based on their respective charged groups. IEX can be used for the separation and purification of charged proteins, small nucleotides, and amino acids.
Cat. No. | Product Name | Matrix | Ionic Capacity | Binding Capacity |
E-CM-IE01 | SP Focurose 6FF | Highly cross-linked 6% agarose | 180-300 µmol H+/mL (media) | 100 mg Lysyme /mL (media) |
E-CM-IE02 | SP Focurose 6HP | Highly cross-linked 6% agarose | 200-300 µmol H+/mL (media) | 120 mg Lysyme /mL (media) |
E-CM-IE03 | SP Focurose HPR | Highly cross-linked agarose and cellulose | 180-280 µmol H+/mL (media) | 100 mg Lysyme /mL (media) |
E-CM-IE04 | SP Focurose 6HF | Highly cross-linked 6% agarose and cellulose | 140-220 µmol H+/mL (media) | 120 mg Lysyme /mL (media) |
E-CM-IE05 | SP Focurose 6XL | Highly cross-linked agarose and dextran | 180-250 µmol H+/mL (media) | >160 mg Lysyme /mL (media) |
E-CM-IE07 | CM Focurose 6FF | Highly cross-linked 6% agarose | 90-150 µmol H+/mL (media) | 110 mg Lysyme /mL (media) |
E-CM-IE08 | CM Focurose 6HP | Highly cross-linked 6% agarose | 90-160 µmol H+/mL (media) | 95 mg Lysyme /mL (media) |
E-CM-IE09 | Q Focurose 6FF | Highly cross-linked 6% agarose | 180-360 µmol Cl-/mL (media) | 90 mg BSA/mL (media) |
E-CM-IE10 | Q Focurose 6HP | Highly cross-linked 6% agarose | 200-280 µmol Cl-/mL (media) | 130 mg BSA/mL (media) |
E-CM-IE11 | Q Focurose 6HPR | Highly cross-linked agarose and cellulose | 180-250 µmol Cl-/mL (media) | 120 mg BSA/mL (media) |
E-CM-IE12 | Q Focurose 6HF | Highly cross-linked 6% agarose and cellulose | 160-220 µmol Cl-/mL (media) | 100 mg BSA/mL (media) |
E-CM-IE13 | Q Focurose 6XL | Highly cross-linked agarose and dextran | 200-350 µmol Cl-/mL (media) | >130 mg BSA/mL (media) |
E-CM-IE15 | DEAE Focurose 6FF | Highly cross-linked 6% agarose | 150-300 µmol Cl-/mL (media) | 110 mg BSA/mL (media) |
E-CM-IE16 | DEAE Focurose 6XL | Highly cross-linked agarose and dextran | 460-510 µmol Cl-/mL (media) | 180 mg BSA/mL (media) |
E-CM-IE17 | DEAE Focurose 6HP | Highly cross-linked 6% agarose | 180-260 µmol Cl-/mL (media) | 90 mg BSA/mL (media) |
E-CM-IE18 | ANX Focurose 6FF | Highly cross-linked 6% agarose | 100-200 µmol Cl-/mL (media) | 80 mg BSA/mL (media) |
Hydrophobic interaction chromatography
Hydrophobic interaction chromatography (HIC) is a useful method for the purification and separation of biomolecules based on their surface hydrophobicity. HIC can be applied to the separation and purification of hydrophobic proteins, as well as aromatic and aliphatic compounds. In HIC, the matrix material is lightly substituted with hydrophobic groups, such as methyl, ethyl, propyl, octyl, or phenyl groups.
Cat. No. | Product Name | Matrix | Ligand Density | Binding Capacity |
E-CM-HI01 | Phenyl Focurose 6FF (HS) | Highly cross-linked 6% agarose | 40-50 µmol/mL | 40 mg (IgG)/mL (media) |
E-CM-HI02 | Phenyl Focurose 6FF (LS) | Highly cross-linked 6% agarose | 15-20 µmol/mL | 15 mg (IgG)/mL (media) |
E-CM-HI03 | Phenyl Focurose 6HP | Highly cross-linked 6% agarose | 25-30 µmol/mL | 30 mg (IgG)/mL (media) |
E-CM-HI06 | Butyl-S Focurose 6FF | Highly cross-linked 6% agarose | 10 µmol/mL | 10-20 mg (Lysyme)/mL (media) |
E-CM-HI07 | Octyl Focurose 4FF | Highly cross-linked 4% agarose | ~5 µmol/mL | ~10 mg (Lysyme)/mL (media) |
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