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Protein Purification & Separation

Source: Elabscience®Published: Feb 05,2024

 

For most researchers in biochemistry, structural biology, and functional proteomics, a large amount of time and energy is devoted to the separation and purification of specific proteins or other biomolecules. This step is one of the most basic and important procedures in experimentation, and it is crucial for the accuracy of laboratory results and the management of experimental costs. Only by performing this process efficiently can we remain competitive with other researchers.

So, who is the 'clutch player' in this 'nonexplosive warfare'? The answer is high-quality chromatographic media. Elabscience® offers an extensive range of chromatographic media for the separation and purification of most biomolecules. We have curated our best products for all types of packing to help you separate and purify your target molecules easily and efficiently. All of these products have been rigorously verified by our skilled technicians, ensuring they are high-quality and can help you reduce costs and achieve favorable results. View our offerings and see how Elabscience® can assist with your biochemical research.

Gel filtration chromatography

Gel filtration chromatography, also known as size exclusion chromatography (SEC), is a chromatographic method that separates molecules based on their size or molecular weight. SEC is used for a wide range of separations and purifications of components with large molecular weight differences and low resolution requirements, such as proteins, polysaccharides, and other macromolecules.

Cat. No. Product Name Matrix Particle Size Range Average Particle Size
E-CM-GF01 Focurose 4B 4% agarose 45-165 µm 90 µm
E-CM-GF02 Focurose 6B 6% agarose 45-165 µm 90 µm
E-CM-GF06 Focurose CL-6B Lowly cross-linked 6% agarose 45-165 µm 90 µm
E-CM-GF07 Focurose 4FF Highly cross-linked 4% agarose 45-165 µm 90 µm
E-CM-GF08 Focurose 6FF Highly cross-linked 6% agarose 45-165 µm 90 µm

 

Affinity chromatography

Affinity chromatography is a powerful technique for separating and purifying proteins based on the highly specific interactions between an antigen and antibody, enzyme and substrate, or receptor and ligand. These interactions include hydrogen bonding, ionic interactions, disulfide bridges, and hydrophobic interactions. Affinity chromatography is widely recognized for its high selectivity, high resolution, and high capacity.

Cat. No. Product Name Matrix Binding Capacity
E-CM-AF03 NHS Focurose 4FF Highly cross-linked 4% agarose 16-23 μmol /mL (media)
E-CM-AF04 GST Focurose 4FF Highly cross-linked 4% agarose 15-25mg (GST-tagged protein)/mL (medium)
E-CM-AF06 Ni Focurose 6FF (TED) Highly cross-linked 6% agarose 20-30 mg (His-tagged protein)/mL (medium)
E-CM-AF07 Ni Focurose 6FF (IMAC) Highly cross-linked 6% agarose 45 mg (His-tagged protein)/mL (medium)
E-CM-AF08 Ni Focurose 6FF (IDA) Highly cross-linked 4% agarose 40-50 mg (His-tagged protein)/mL (medium)
E-CM-AF13 Benzamidine Focurose 4FF(HS) Highly cross-linked 4% agarose 25-50 mg (Trypsase)/mL (media)
E-CM-AF17 CNBr Focurose 4FF Highly cross-linked 4% agarose 30 mg(Trypsinogen)/mL(media)
E-CM-AF18 Epoxy Focurose 4FF Highly cross-linked 4% agarose 25 μmol /mL(media)

 

Ion exchange chromatography

Ion exchange chromatography (IEX) is a practical chromatography technique that separates ions and polar molecules based on their respective charged groups. IEX can be used for the separation and purification of charged proteins, small nucleotides, and amino acids.

Cat. No. Product Name Matrix Ionic Capacity Binding Capacity
E-CM-IE01 SP Focurose 6FF Highly cross-linked 6% agarose 180-300 µmol H+/mL (media) 100 mg Lysyme /mL (media)
E-CM-IE02 SP Focurose 6HP Highly cross-linked 6% agarose 200-300 µmol H+/mL (media) 120 mg Lysyme /mL (media)
E-CM-IE03 SP Focurose HPR Highly cross-linked agarose and cellulose 180-280 µmol H+/mL (media) 100 mg Lysyme /mL (media)
E-CM-IE04 SP Focurose 6HF Highly cross-linked 6% agarose and cellulose 140-220 µmol H+/mL (media) 120 mg Lysyme /mL (media)
E-CM-IE05 SP Focurose 6XL Highly cross-linked agarose and dextran 180-250 µmol H+/mL (media) >160 mg Lysyme /mL (media)
E-CM-IE07 CM Focurose 6FF Highly cross-linked 6% agarose 90-150 µmol H+/mL (media) 110 mg Lysyme /mL (media)
E-CM-IE08 CM Focurose 6HP Highly cross-linked 6% agarose 90-160 µmol H+/mL (media) 95 mg Lysyme /mL (media)
E-CM-IE09 Q Focurose 6FF Highly cross-linked 6% agarose 180-360 µmol Cl-/mL (media) 90 mg BSA/mL (media)
E-CM-IE10 Q Focurose 6HP Highly cross-linked 6% agarose 200-280 µmol Cl-/mL (media) 130 mg BSA/mL (media)
E-CM-IE11 Q Focurose 6HPR Highly cross-linked agarose and cellulose 180-250 µmol Cl-/mL (media) 120 mg BSA/mL (media)
E-CM-IE12 Q Focurose 6HF Highly cross-linked 6% agarose and cellulose 160-220 µmol Cl-/mL (media) 100 mg BSA/mL (media)
E-CM-IE13 Q Focurose 6XL Highly cross-linked agarose and dextran 200-350 µmol Cl-/mL (media) >130 mg BSA/mL (media)
E-CM-IE15 DEAE Focurose 6FF Highly cross-linked 6% agarose 150-300 µmol Cl-/mL (media) 110 mg BSA/mL (media)
E-CM-IE16 DEAE Focurose 6XL Highly cross-linked agarose and dextran 460-510 µmol Cl-/mL (media) 180 mg BSA/mL (media)
E-CM-IE17 DEAE Focurose 6HP Highly cross-linked 6% agarose 180-260 µmol Cl-/mL (media) 90 mg BSA/mL (media)
E-CM-IE18 ANX Focurose 6FF Highly cross-linked 6% agarose 100-200 µmol Cl-/mL (media) 80 mg BSA/mL (media)

 

Hydrophobic interaction chromatography

Hydrophobic interaction chromatography (HIC) is a useful method for the purification and separation of biomolecules based on their surface hydrophobicity. HIC can be applied to the separation and purification of hydrophobic proteins, as well as aromatic and aliphatic compounds. In HIC, the matrix material is lightly substituted with hydrophobic groups, such as methyl, ethyl, propyl, octyl, or phenyl groups.

Cat. No. Product Name Matrix Ligand Density Binding Capacity
E-CM-HI01 Phenyl Focurose 6FF (HS) Highly cross-linked 6% agarose 40-50 µmol/mL 40 mg (IgG)/mL (media)
E-CM-HI02 Phenyl Focurose 6FF (LS) Highly cross-linked 6% agarose 15-20 µmol/mL 15 mg (IgG)/mL (media)
E-CM-HI03 Phenyl Focurose 6HP Highly cross-linked 6% agarose 25-30 µmol/mL 30 mg (IgG)/mL (media)
E-CM-HI06 Butyl-S Focurose 6FF Highly cross-linked 6% agarose 10 µmol/mL 10-20 mg (Lysyme)/mL (media)
E-CM-HI07 Octyl Focurose 4FF Highly cross-linked 4% agarose ~5 µmol/mL ~10 mg (Lysyme)/mL (media)

 

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