Elabscience® Cell Viability Chemiluminescence Assay Kit, Fast and Accurate Detection of Cell Viability

When performing cell proliferation or toxicity testing, are you often troubled by issues like high background signals, poor sample uniformity, time-consuming procedures, or inconsistent results? Don't worry, Elabscience^® next-generation cell viability detection product, Cell Viability Chemiluminescence Assay Kit, is here!

The core advantage of the chemiluminescence method lies in its "glow mechanism"—no excitation light is needed. Instead, it relies on specific chemical reactions to release photons, inherently avoiding the common pitfalls of fluorescence-based assays. Compared to traditional MTT, CCK-8 colorimetric, and fluorescence methods, chemiluminescence delivers superior precision and efficiency, outperforming conventional techniques.

Table of Contents

1. Detection Principle of the Cell Viability Detection Product

2. Product Features of the Cell Viability Detection Product

3. Performance Data of the Cell Viability Detection Product

4. Comprehensive Assay Solutions for Cell Metabolism Studies

01 Detection Principle of the Cell Viability Detection Product

This product enables cell viability detection based on the ATP luminescence method. The core mechanism is as follows: ATP is a key energy metabolite in oxidative phosphorylation, and changes in its concentration reflect changes in cell viability.

Released ATP acts as a specific substrate for luciferase, triggering a cascade of enzymatic reactions with luciferase and luciferin. This reaction produces a chemiluminescent signal that can be captured by a reader. The luminescent signal shows an excellent linear relationship with ATP concentration in the sample, which directly corresponds to the number of viable cells before lysis. Thus, by detecting the luminescence intensity, cell viability can be accurately quantified.

02 Product Features of the Cell Viability Detection Product

2.1 High Sensitivity & Broad Cell Linearity

Offers a wide linear detection range of 500-50,000 cells, making it suitable for both high and low viability cell types, including rare or limited samples.

2.2 Fast and Easy to Use

Simple reagent preparation: just add the working solution and read. No waiting required, making it faster and more convenient than traditional methods like MTT or CCK-8. 

2.3 High Adaptability

Compatible with cells harvested from culture dishes or directly seeded in microplates, meeting diverse experimental needs.

03 Performance Data of the Cell Viability Detection Product

The kit demonstrates excellent linearity in detecting both ATP standards and cell samples.

Fig. 1 Linearity of ATP detection using Cell Viability Chemiluminescence Assay Kit (E-BC-F200).

Fig. 2 Linearity of Hela cell detection using Cell Viability Chemiluminescence Assay Kit (E-BC-F200).

Hela cells treated with 0.2 μM and 2 μM antimycin A for 1 hour showed expected trends in cell viability compared to untreated cells when measured with Cell Viability Chemiluminescence Assay Kit (E-BC-F200).

Fig. 3 Cell viability measurement of inhibitor-treated Hela cells using Cell Viability Chemiluminescence Assay Kit (E-BC-F200).

04 Comprehensive Assay Solutions for Cell Metabolism Studies

Elabscience^® offers a range of related products to support multidimensional experimental designs in cell metabolism and function research.

Table 1. Cell Viability and Metabolic Assay Kits Overview

With its streamlined protocol, the chemiluminescence-based cell viability assay kit enables rapid and accurate assessment of cell viability. Stay tuned for more new cell metabolism assay products from Elabscience^®!