Unlike monoclonal antibodies, polyclonal antibodies correspond to multiple epitopes and tend to contain several immunoglobulin subtypes. They can be used as both primary and secondary antibodies in Westerns, ELISAs, and IHCs.
Elabscience® provides polyclonal antibody services in mouse, rat, rabbit, and other species. Our polyclonal antibody packages include antigen design, peptide synthesis, immunization, purification, ELISA test, and lyophilization. You can simply select your desired packages and send us the specific requirements, and then Elabscience® will offer antibodies with guaranteed quality.
Advantages
Multiple antibody labeling for
alternatives: Elabscience® offers multiple antibody labeling for you to choose, including Biotin,
HRP, FITC, Cy3, Cy5, APC, etc.
Flexible service portfolio: Different host species (mouse,
rat, and rabbit) and purification method (Protein A/G purification/ Antigen
Affinity purification) are available for your research demands.
Personalized experiment scheme: Elabscience’s
experienced antibody experts will design the personalized experiment scheme
according to your specific requirements and applications.
Polyclonal Antibody Services Details
|
Cat.No.
|
Service Name
|
Timeline
|
Quoted price
|
Note
|
|
S-CA-P001
|
Peptide synthesis and conjugation
|
3 weeks
|
$16*(peptide length)*number+150$
|
<15AA, purity >85%, 10 mg
|
|
S-CA-P002
|
Protein expression
|
6-7weeks
|
Depend on the protein
|
|
|
S-CA-P003
|
Polyclonal antisera from rabbit
|
12 weeks
|
385$
|
|
|
S-CA-P004
|
Polyclonal antisera from mouse
|
12 weeks
|
308$
|
|
|
S-CA-P005
|
Polyclonal antisera from rat
|
12 weeks
|
385$
|
|
|
S-CA-P010
|
Protein A/G purification
|
1 week
|
230$
|
|
|
S-CA-P011
|
Antigen Affinity purification
|
1 week
|
385$
|
|
|
S-CA-P012
|
Antibody labeling (Biotin)
|
1 day
|
185$
|
This price is for 1mg marked antibody.
The total price will increase by $120 when the quantity of marked antibody
increase by 1mg.
|
|
S-CA-P013
|
Antibody labeling (HRP)
|
2 days
|
308$
|
|
S-CA-P014
|
Antibody labeling (FITC)
|
1 day
|
185$
|
|
S-CA-P015
|
Antibody labeling (Cy3)
|
1 day
|
230$
|
|
S-CA-P016
|
Antibody labeling (Cy5)
|
1 day
|
230$
|
|
S-CA-P017
|
Antibody labeling (APC)
|
2 days
|
230$
|
In this case,
customer provides purified antigen, we prepare antibody and identified it by
western blot, then determine titer by ELISA and test concentration by BCA.
Result
1. Result of Western blot
As the expression information of target
protein was not told, we identified the antibody with immunogen. The sample
amount is 1.5 μg.
The main band
was at the position of 55~72 kDa and a few unpurposed bands showed up. That may
due to some unpurposed protein exist among the antigenic protein.
2. Result of ELISA
The coated
antigen was 0.2 μg. We detected antisera and purified antibody respectively with
dilution factor 8×104, 16×104, 32×104, 64×104, 128×104, 256×104, 512×104, 512×104.The 3 times of the
blank OD value was regarded as the titer of the antibody.
2.1 Result
of antisera
|
|
Rabbit A
|
Rabbit B
|
Dilution
factor
|
|
|
A
|
0.050
|
0.050
|
0.052
|
0.049
|
Blank
|
|
|
B
|
0.057
|
0.053
|
0.053
|
0.055
|
Negative
control 1/104
|
|
|
C
|
0.098
|
0.107
|
0.138
|
0.125
|
1/256×104
|
|
|
D
|
0.165
|
0.163
|
0.215
|
0.188
|
1/128×104
|
|
|
E
|
0.293
|
0.297
|
0.349
|
0.356
|
1/ 64×104
|
|
|
F
|
0.489
|
0.466
|
0.616
|
0.602
|
1/32×104
|
|
|
G
|
0.894
|
0.916
|
1.065
|
1.088
|
1/16×104
|
|
|
H
|
1.207
|
1.262
|
1.599
|
1.625
|
1/8×104
|
|
2.2 Result of
purified antibody
|
|
Rabbit A
|
Rabbit B
|
Dilution
factor
|
|
A
|
0.06
|
0.066
|
0.061
|
0.062
|
Blank
|
|
B
|
0.08
|
0.069
|
0.072
|
0.082
|
Negative control 1/104
|
|
C
|
0.093
|
0.096
|
0.125
|
0.121
|
1/1024×104
|
|
D
|
0.134
|
0.127
|
0.15
|
0.152
|
1/512×104
|
|
E
|
0.158
|
0.163
|
0.263
|
0.26
|
1/256×104
|
|
F
|
0.274
|
0.275
|
0.479
|
0.481
|
1/128×104
|
|
G
|
0.42
|
0.422
|
0.82
|
0.816
|
1/ 64×104
|
|
H
|
0.695
|
0.691
|
1.356
|
1.355
|
1/32×104
|
The titer of
rabbit A is 128×104. The titer of rabbit B is 256×104.
The calculated concentration
of rabbit A is 24 mg/mL.
The calculated concentration
of rabbit B is 29.9 mg/mL.
3. Result of BCA
Dilute BSA with TE to
constitute concentration gradient 0 mg/mL, 0.2 mg/mL, 0.4 mg/mL, 0.8 mg/mL, 1 mg/mL.
Do duplicate and make standard curve. Dilute purified IgG 10 times, 20 times, 40
times with TE. Do duplicate (The volume of BCA is 200 μL, the volume of protein
is 20 μL), keep at 37℃ for 30 min, read at 568 nm.
|
|
BSA concentration (mg/mL)
|
OD568nm
|
IgG dilution factor
|
|
BSA
|
IgG
|
|
Rabbit A IgG
|
0
|
0.084
|
0.081
|
0.585
|
0.603
|
1/40
|
|
0.2
|
0.283
|
0.281
|
1.02
|
1.054
|
1/20
|
|
0.4
|
0.453
|
0.469
|
1.681
|
1.724
|
1/10
|
|
Rabbit B IgG
|
0.6
|
0.605
|
0.593
|
0.698
|
0.72
|
1/40
|
|
0.8
|
0.748
|
0.732
|
1.145
|
1.184
|
1/20
|
|
1
|
0.897
|
0.896
|
1.818
|
1.861
|
1/10
|
4.
Delivery
|
|
Concentration
|
Volume
|
Titer
|
Components
|
Amount
|
Antisera
|
Negative
serum
|
|
Rabbit
A IgG
|
24
mg/mL
|
1
mL
|
128×104
|
50%
glycerinum, 2 mM EDTA, 0.02%
P300
|
4
vials
|
400 μL
|
100 μL
|
|
Rabbit
B IgG
|
29.9
mg/mL
|
1
mL
|
256×104
|
50%
glycerinum, 2 mM
EDTA, 0.02%
P300
|
6
vials
|
400 μL
|
100 μL
|
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