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Polyclonal Antibody Service
Unlike monoclonal antibodies, polyclonal antibodies correspond to multiple epitopes and tend to contain several immunoglobulin subtypes. They can be used as both primary and secondary antibodies in Westerns, ELISAs, and IHCs.
Elabscience® provides polyclonal antibody services in mouse, rat, rabbit, and other species. Our polyclonal antibody packages include antigen design, peptide synthesis, immunization, purification, ELISA test, and lyophilization. You can simply select your desired packages and send us the specific requirements, and then Elabscience® will offer antibodies with guaranteed quality.
Advantages
Multiple antibody labeling for alternatives: Elabscience® offers multiple antibody labeling for you to choose, including Biotin, HRP, FITC, Cy3, Cy5, APC, etc.
Flexible service portfolio: Different host species (mouse,
rat, and rabbit) and purification method (Protein A/G purification/ Antigen
Affinity purification) are available for your research demands.
Personalized experiment scheme: Elabscience’s experienced antibody experts will design the personalized experiment scheme according to your specific requirements and applications.
Polyclonal Antibody Services Details
Cat.No.
Service Name
Timeline
Quoted price
Note
S-CA-P001
Peptide synthesis and conjugation
3 weeks
$16*(peptide length)*number+150$
<15AA, purity >85%, 10 mg
S-CA-P002
Protein expression
6-7weeks
Depend on the protein
S-CA-P003
Polyclonal antisera from rabbit
12 weeks
385$
S-CA-P004
Polyclonal antisera from mouse
12 weeks
308$
S-CA-P005
Polyclonal antisera from rat
12 weeks
385$
S-CA-P010
Protein A/G purification
1 week
230$
S-CA-P011
Antigen Affinity purification
1 week
385$
S-CA-P012
Antibody labeling (Biotin)
1 day
185$
This price is for 1mg marked antibody.
S-CA-P013
Antibody labeling (HRP)
2 days
308$
S-CA-P014
Antibody labeling (FITC)
1 day
185$
S-CA-P015
Antibody labeling (Cy3)
1 day
230$
S-CA-P016
Antibody labeling (Cy5)
1 day
230$
S-CA-P017
Antibody labeling (APC)
2 days
230$
The total price will increase by $120 when the quantity of marked antibody
increase by 1mg.
In this case, customer provides purified antigen, we prepare antibody and identified it by western blot, then determine titer by ELISA and test concentration by BCA.
Result
1. Result of Western blot
As the expression information of target protein was not told, we identified the antibody with immunogen. The sample amount is 1.5 μg.
The main band was at the position of 55~72 kDa and a few unpurposed bands showed up. That may due to some unpurposed protein exist among the antigenic protein.
2. Result of ELISA
The coated antigen was 0.2 μg. We detected antisera and purified antibody respectively with dilution factor 8×104, 16×104, 32×104, 64×104, 128×104, 256×104, 512×104, 512×104.The 3 times of the blank OD value was regarded as the titer of the antibody.
2.1 Result of antisera
|
|
Rabbit A |
Rabbit B |
Dilution factor |
|
||
|
A |
0.050 |
0.050 |
0.052 |
0.049 |
Blank |
|
|
B |
0.057 |
0.053 |
0.053 |
0.055 |
Negative control 1/104 |
|
|
C |
0.098 |
0.107 |
0.138 |
0.125 |
1/256×104 |
|
|
D |
0.165 |
0.163 |
0.215 |
0.188 |
1/128×104 |
|
|
E |
0.293 |
0.297 |
0.349 |
0.356 |
1/ 64×104 |
|
|
F |
0.489 |
0.466 |
0.616 |
0.602 |
1/32×104 |
|
|
G |
0.894 |
0.916 |
1.065 |
1.088 |
1/16×104 |
|
|
H |
1.207 |
1.262 |
1.599 |
1.625 |
1/8×104 |
|
2.2 Result of purified antibody
|
|
Rabbit A |
Rabbit B |
Dilution factor |
||
|
A |
0.06 |
0.066 |
0.061 |
0.062 |
Blank |
|
B |
0.08 |
0.069 |
0.072 |
0.082 |
Negative control 1/104 |
|
C |
0.093 |
0.096 |
0.125 |
0.121 |
1/1024×104 |
|
D |
0.134 |
0.127 |
0.15 |
0.152 |
1/512×104 |
|
E |
0.158 |
0.163 |
0.263 |
0.26 |
1/256×104 |
|
F |
0.274 |
0.275 |
0.479 |
0.481 |
1/128×104 |
|
G |
0.42 |
0.422 |
0.82 |
0.816 |
1/ 64×104 |
|
H |
0.695 |
0.691 |
1.356 |
1.355 |
1/32×104 |
The titer of rabbit A is 128×104. The titer of rabbit B is 256×104.
The calculated concentration of rabbit A is 24 mg/mL.
The calculated concentration of rabbit B is 29.9 mg/mL.
3. Result of BCA
Dilute BSA with TE to constitute concentration gradient 0 mg/mL, 0.2 mg/mL, 0.4 mg/mL, 0.8 mg/mL, 1 mg/mL. Do duplicate and make standard curve. Dilute purified IgG 10 times, 20 times, 40 times with TE. Do duplicate (The volume of BCA is 200 μL, the volume of protein is 20 μL), keep at 37℃ for 30 min, read at 568 nm.
|
|
BSA concentration (mg/mL) |
OD568nm |
IgG dilution factor |
|||
|
BSA |
IgG |
|||||
|
Rabbit A IgG |
0 |
0.084 |
0.081 |
0.585 |
0.603 |
1/40 |
|
0.2 |
0.283 |
0.281 |
1.02 |
1.054 |
1/20 |
|
|
0.4 |
0.453 |
0.469 |
1.681 |
1.724 |
1/10 |
|
|
Rabbit B IgG |
0.6 |
0.605 |
0.593 |
0.698 |
0.72 |
1/40 |
|
0.8 |
0.748 |
0.732 |
1.145 |
1.184 |
1/20 |
|
|
1 |
0.897 |
0.896 |
1.818 |
1.861 |
1/10 |
|
4. Delivery
|
|
Concentration |
Volume |
Titer |
Components |
Amount |
Antisera |
Negative serum |
|
Rabbit A IgG |
24 mg/mL |
1 mL |
128×104 |
50% glycerinum, 2 mM EDTA, 0.02% P300 |
4 vials |
400 μL |
100 μL |
|
Rabbit B IgG |
29.9 mg/mL |
1 mL |
256×104 |
50% glycerinum, 2 mM EDTA, 0.02% P300 |
6 vials |
400 μL |
100 μL |
