Nitric Oxide (NO) is more than just a signaling molecule. It's a true regulator:
Cardiovascular system: Controls vasodilation and blood pressure
Immune system: Frontline defender against pathogens
Nervous system: Mediates neurotransmission and synaptic plasticity
But traditional NO detection kits often come with headaches:
(1) Complicated procedures
(2) Low sensitivity
(3) No live-cell dynamic monitoring
(4) Poor compatibility with complex samples
Elabscience® has solved them all.
Elabscience® expands its NO detection family with two powerful new kits –Nitric Oxide (NO) Colorimetric Assay Kit (E-BC-K817-M) and Nitric Oxide (NO) Fluorometric Assay Kit (E-BC-F021) – joining our classic bestsellers Nitric Oxide (NO) Colorimetric Assay Kit (E-BC-K035-M), Nitric Oxide (NO) Colorimetric Assay Kit (Nitrate Reductase Method) (E-BC-K135-M), and Nitric Oxide (NO) Colorimetric Assay Kit (E-BC-K035-S). Together, they deliver a high-compatibility, high-throughput, multi-platform precision solution for NO detection.
Table of Contents
1. Introducing next-generation Nitric Oxide detection solutions
2. Key technical advantages for Nitric Oxide analysis
3. Analytical performance validation and experimental data
4. Choosing the right Nitric Oxide detection strategy for your research
01 Introducing next-generation Nitric Oxide detection solutions
1.1 E-BC-K817-M: The Efficient and Convenient Choice
Tackling the complexity of traditional methods, this kit uses an innovative chemical reduction method that enables direct microplate detection – no transfer or centrifugation steps. The workflow is dramatically simplified, speeding up your NO quantification.
Best for: High-throughput, multi-sample NO quantification where efficiency matters.
_.png)
Fig. 1 Schematic of the Nitric Oxide (NO) Colorimetric Assay Kit (E-BC-K817-M).
1.2 E-BC-F021: Real-Time Live-Cell Monitoring
Designed for low-concentration NO in cell samples and the challenge of capturing dynamic changes, this kit employs DAF-FM DA fluorescent probe to directly monitor real-time NO production in living cells – not just metabolic byproducts.
Best for: Cell biology studies requiring live-cell NO dynamics.
02 Key technical advantages for Nitric Oxide analysis
Fast & simple: One-step microplate assay; no EP tubes or centrifugation
Wide linear range & high sensitivity: 0.46 -100 μmol/L
Broad sample compatibility: Serum, plasma, urine, tissue, and cell sample
Comprehensive metabolic insight: Quantifies total NO (NO₃⁻ + NO₂⁻), overcoming the limitation of traditional methods that detect only nitrite, making it ideal for drug screening.
03 Analytical performance validation and experimental data
%20Colorimetric%20Assay%20Kit%20(E-BC-K817-M)_.png)
Fig. 2 Standard curve of the Nitric Oxide (NO) Colorimetric Assay Kit (E-BC-K817-M).
%20Colorimetric%20Assay%20Kit%20(E-BC-K817-M)_.png)
Fig. 3 Detection of nitrate and nitrite standards using the Nitric Oxide (NO) Colorimetric Assay Kit (E-BC-K817-M).
%20measured%20with%20the%20Nitric%20Oxide%20(NO)%20Colorimetric%20Assay%20Kit%20(E-BC-K817-M)_.png)
Fig. 4 NO levels in Raw264.7 cells (M1 vs. M0) measured with the Nitric Oxide (NO) Colorimetric Assay Kit (E-BC-K817-M).
04 Choosing the right Nitric Oxide detection strategy for your research
From micro to routine, from classic nitrate reductase to upgraded chemical reduction, from colorimetric to fluorescent, from high-throughput to flexible validation – our 5-product matrix covers all your NO detection requirements.
Table 1. Selecting the appropriate Nitric Oxide (NO) Assay Kit
|
Cat. No. |
Analyte |
Method |
Recommended Samples |
Key Advantage |
|
E-BC-F021 (NEW) |
NO |
Fluorescence |
Cells, low-concentration NO cell samples |
Live-cell detection, ultra-high sensitivity |
|
E-BC-K817-M (NEW) |
NO3- + NO2- |
Chemical reduction |
Serum, plasma, urine, tissue, cells |
Low sample volume, microplate-ready, wide sample compatibility |
|
E-BC-K135-M |
NO3- + NO2- |
Nitrate reductase |
Serum, plasma, urine, tissue, cells, cell supernatant |
Broad sample coverage |
|
E-BC-K035-M |
NO2- |
Griess method |
Serum, plasma, animal/plant tissues |
High sensitivity, low cost, ideal for screening |
|
E-BC-K035-S |
NO2- |
Griess method |
Serum, plasma, animal/plant tissues |
Low instrument requirement, low cost, screening-ready |
Whether you need high-throughput screening, live-cell dynamic monitoring, or classic method validation – Elabscience® has you covered.
Two new leads, five powerful solutions, whatever your sample, instrument, or research goal, there's a perfect match waiting for you.

