Nitric Oxide (NO) Colorimetric Assay Kit (E-BC-K817-M)
For research use only.
| Detection Principle | Nitric oxide (NO) is chemically reactive and is rapidly metabolized in the body to nitrite (NO2- ) and nitrate ((NO3-), with NO2- further converting to NO3-. This method utilizes a reducing agent to convert NO3- to NO2-, and the color development with a chromogenic reagent can be detected at 540 nm to determine the content of NO. |
| Sample Type | Serum,Plasma,Urine,Animal tissue,Cell |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (530-550 nm) |
| Other Reagents Required | Double distilled water, 1×PBS (0.01 M, pH 7.4), 2 M NaOH |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.46 μmol/L |
| Detection Range | 0.46-100.00 μmol/L |
| Precision | inter-assay CV: 3.4%-4.8% | intra-assay CV: 1.0%-4.7% |
| Sample Volume | 150-300 μL |
| Assay Time | 1 h 20 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Mouse liver tissue homogenization | 1 |
| 10% Mouse kidney tissue homogenization | 20-30 |
| 10% Mouse heart tissue homogenization | 1 |
| 10% Mouse spleen tissue homogenization | 1 |
| Rats serum | 1 |
| Human plasma | 1 |
| Human urine | 1 |
| 1×10^6 HL-60 cells | 1 |
| 1×10^6 H9c2 cells | 1 |
| 1×10^6 Hela cells | 1 |
| 1×10^6 Jurkat cells | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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