Nitric Oxide (NO) Fluorometric Assay Kit (E-BC-F021)
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For research use only.
| Detection Principle |
Nitric oxide (NO) is a nonpolar free radical gas molecule and serves as a key participant in biological signal transduction. It can rapidly diffuse and transmit signals between cells, playing important roles in promoting vasodilation, facilitating neurotransmission, and regulating immune and metabolic functions. DAF-FM DA probe can enter cells and react with NO to produce fluorescence. The NO levels in the cell can be evaluated by measuring the fluorescence intensity. |
| Sample Type | Cell |
| Detection Method | Fluorometric method |
| Detection Instrument | Flow Cytometry |
| Other Reagents Required | Phenol red-free basal medium or phenol red-free Hank's balanced salt solution (Hank's), PBS (0.01 M, pH 7.4) |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Cat.No. | Product Name | Clone No. |
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