AA(Arachidonic Acid) ELISA Kit (E-EL-0051)

Name: AA(Arachidonic Acid) ELISA Kit
Brand: Elabscience
SKU: E-EL-0051
Price: 150.0 USD
Availability: InStock

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Size	Price	Qty
96T	$ 495.00	- +
48T	$ 396.00	- +
24T	$ 150.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	0.94 ng/mL
Detection Range	1.56-100 ng/mL
Sample Volume	50 μL
Total Assay Time	2 h 30 min
Reactivity	Universal
Specificity	This kit recognizes Universal AA in samples.No significant cross-reactivity or interference between Universal AA and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Colorimetric method, ELISA, Competitive
Assay Type	Competitive-ELISA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃
Expiration Date	12 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

ng/mL	OD1	OD2	Mean OD	Corrected OD
100	0.236	0.236	0.236	-
50	0.344	0.356	0.350	-
25	0.582	0.576	0.579	-
12.5	0.861	0.889	0.875	-
6.25	1.309	1.265	1.287	-
3.13	1.630	1.646	1.638	-
1.56	1.838	1.892	1.865	-
0	2.239	2.243	2.241	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（ng/mL）	5.080	12.660	37.060	4.870	13.400	40.650
Standard deviation	0.230	0.820	2.290	0.410	0.940	2.820
CV（%）	4.520	6.440	6.190	8.410	7.040	6.930

Recovery

The recovery of Universal AA was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	87-98	93
EDTA plasma （n=8）	93-110	101
Cell culture media （n=8）	90-103	98

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Universal AA and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	93-108	100-111	98-112
1:2	Average	99	105	105
1:4	Range	95-111	90-103	88-101
1:4	Average	102	97	93
1:8	Range	94-111	91-103	87-98
1:8	Average	102	97	92

Stability

Each kit batch is subjected to accelerated stability testing and real-time stability monitoring. Sample performance is evaluated after storage at 37 °C for 10 days to assess the impact of elevated temperature on assay reliability and reagent integrity.

/	Variation range of 37°C mean concentration / 2-8°C mean
Sample 1（n=16）	86.15-97.69
Sample 2（n=16）	89.64-110.41

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal AA. During the reaction, Universal AA in the sample or standard competes with a fixed amount of Universal AA on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal AA. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal AA in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Background

Arachidonic Acid, also known as arachidonic acid (AA or ARA), is an important polyunsaturated fatty acid. Its chemical name is pancis-5,8,11,14-eicosatetraenoic acid (CIS-5,8,11,14-eicosatetraenoic acid), the molecular formula is C20H32O2, the molecular weight is 304.46, and the CAS number is 506-32-1. Arachidonic acid is liquid at room temperature, with a melting point of -49.5 ° C and a boiling point of 245 ° C, and can be dissolved in alcohols, ethers and water. It is widely distributed in the neutral fat of animals, is one of the most abundant and widely distributed polyunsaturated fatty acids in mammals, and is also an important essential fatty acid in human body. Arachidonic acid can produce many metabolites such as prostaglandin, throboxane, lipoxygenin, leukotriene, hydroxy-eicosatetraenoic acid and epoxide eicosatetraenoic diol through three metabolic pathways regulated by cyclooxygenase (COX), lipoxygenase (LOX) and cytochrome P450 (CYP450). These metabolites are widely involved in organ function maintenance, inflammatory response, drug metabolism and other physiological processes, and play an important role in the occurrence and development of cardiovascular diseases, cancer and other diseases

Research Area

Signaltransduction