For research use only.
| Detection Principle | Aconitase (ACO) is an important Fe/S protein enzyme in cells, which mainly exists in cytoplasm and mitochondria. ACO catalyzes the reversible reaction of citric acid to isocitric acid from the intermediate product cisaconite acid in the cell, which plays an important role through maintaining the tricarboxylic acid cycle and glyoxylic acid cycle. Aconitase catalyzes isocitrate to produce cis-aconitase. Cis-aconitase has a characteristic absorption peak at 240 nm. The activity of this enzyme was calculated by measuring the production rate of cis-aconitase. |
| Synonyms | ACO |
| Sample Type | Animal tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (230-250 nm, optimum wavelength: 240 nm) |
| Research Area | Tricarboxylic Acid（TCA）Cycle , Energy Metabolism |
| Other Reagents Required | Normal saline (0.9% NaCl) , PBS (0.01 mol/L, pH 7.4) |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 2.32 U/L |
| Detection Range | 2.32-60.19 U/L |
| Precision | inter-assay CV: 9.5% | intra-assay CV: 5.9% |
| Assay Time | 25 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Rat liver tissue homogenate | 6-10 |
| 10% Mouse kidney tissue homogenate | 4-6 |
| 10% Mouse liver tissue homogenate | 6-10 |
| 10% Mouse brain tissue homogenate | 4-6 |
| 10% Rat lung tissue homogenate | 6-10 |
| 10% Mouse muscle tissue homogenate | 2-4 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
Other Clones
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Biotin
Chromogenic Biotin
Cyanine 3
Cyanine 5
Cyanine 5.5
Cyanine 7
Elab Fluor®405
Elab Fluor®430
Elab Fluor®488
Elab Fluor®568
Elab Fluor®594
Elab Fluor®647
Elab Fluor®680
Elab Fluor®700
Elab Fluor®750
Elab Fluor®Red 780
Elab Fluor®Violet 450
FITC
HRP
NHS-Biotin
NHS-LC-LC-Biotin
Sulfo-NHS-Biotin
Sulfo-NHS-LC-LC-Biotin
Unconjugated
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