Aconitase catalyzes isocitrate to produce cis-aconitase. Cis-aconitase has a characteristic absorption peak at 240 nm. The activity of this enzyme was calculated by measuring the production rate of cis-aconitase.

Performance characteristics

Synonyms	ACO
Sample type	animal tissue
Sensitivity	2.32 U/L
Detection range	2.32-60.19 U/L
Detection method	Colorimetric method
Assay type	Enzyme Activity
Assay time	25 min
Precision	Average inter-assay CV: 9.500%Average intra-assay CV: 5.900%
Other instruments required	Centrifuge
Other reagents required	Normal saline (0.9% NaCl) , PBS(0.01 M，pH 7.4)
Storage	-20℃
Valid period	12 months

Ralated product

Cat Number	Product Name
E-BC-K650-M	Pyruvate Dehydrogenase (PDH) Activity Assay Kit
E-BC-K178-M	Citrate Synthase(CS) Activity Assay Kit
E-BC-K652-M	Acetyl-CoA Colorimetric Assay Kit
E-BC-K083-M	α-Ketoglutarate Dehydrogenase(α-KGDH) Activity Assay Kit
E-BC-K651-M	NAD-Isocitrate Dehydrogenase (NAD-IDH) Activity Assay Kit

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

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Cat. No	E-BC-K656-M
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