Pyruvate Dehydrogenase (PDH) Activity Assay Kit (E-BC-K650-M)
For research use only.
| Detection Principle | Pyruvate dehydrogenase (PDH) catalyzed the oxidative decarylation of pyruvate into acetyl CoA, which plays an important role in the metabolism of mitochondrial respiratory chain and is associated with the aging of cancer genes. Pyruvate can be decarboxylated by PDH under the action of CoA. Meanwhile, NAD+ is reduced to NADH, Which under the action of hydrogen transmitter, transfer electrons to WST-8 to produce the yellow product. The activity of PDH can be calculated by measuring the change of absorbance value at 450 nm. |
| Synonyms | PDH |
| Sample Type | Serum,Plasma,Urine,Animal tissue,Cell |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (440-460 nm, optimum wavelength: 450 nm) |
| Research Area | Tricarboxylic Acid（TCA）Cycle , Energy Metabolism |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.28 U /L |
| Detection Range | 0.28-168.91 U/L |
| Precision | inter-assay CV: 0.1 | intra-assay CV: 0.02 |
| Sample Volume | 40 μL |
| Assay Time | 30 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Rat liver tissue homogenate | 2-3 |
| 10% Rat kidney tissue homogenate | 1 |
| 10% Rat heart tissue homogenate | 1 |
| 10% Rat brain tissue homogenate | 1 |
| 10% Rat lung tissue homogenate | 1 |
| 10% Mouse liver tissue homogenate | 2-3 |
| 10% Mouse kidney tissue homogenate | 1 |
| 10% Mouse lung tissue homogenate | 1 |
| 1 × 10^6 HL-60 cells | 1 |
| Rat serum | 1 |
| Rat plasma | 1 |
| Human urine | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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