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Current Position:Home>>Antibodies>>Flow Cytometry Antibodies>> Elab Fluor® 488 Anti-Human CD45RO Antibody[UCHL1]

Elab Fluor® 488 Anti-Human CD45RO Antibody[UCHL1]

  • Cat.No.:E-AB-F1139L

  • Applications: FCM
  • Isotype: Mouse IgG2a, κ
  • Host: Mouse
Applications:

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  • 20Tests
  • 100Tests
  • 100Tests×2
Price: $62
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Antigen Details

References
1. Thomas M. 1989. Annu. Rev. Immunol. 7:339.
2. Trowbridge I, et al. 1994. Annu. Rev. Immunol. 12:85.

Product Details

Form Liquid
Clone No. UCHL1(See Other Available Formats)
Host Mouse
Isotype Mouse IgG2a, κ
Isotype Control Elab Fluor® 488 Mouse IgG2a, κ Isotype Control[C1.18.4] [Product E-AB-F09802L]
Reactivity Human
Application FCM (See the Application Description)
Storage Buffer Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Shipping Biological ice pack at 4℃
Stability & Storage Keep as concentrated solution.
Store at 2~8°C and protected from prolonged exposure to light. Do not freeze.
Centrifuge before opening to ensure complete recovery of vial contents.
This product is guaranteed up to one year from purchase.
Conjugation Elab Fluor® 488
Elab Fluor® 488 is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 520 nm (e.g., a 525/40 nm bandpass filter).


How to select the appropriate detection channel through the spectrogram?

Background

CD45RO is a 180 kD single chain membrane glycoprotein. It is a splice variant of tyrosine phosphatase CD45, lacking the A, B, and C determinants. The CD45RO isoform is expressed on activated and memory T cells, some B cell subsets, activated monocytes/macrophages, and granulocytes. CD45RO enhances both T cell receptor and B cell receptor signaling mediated activation. CD45 and its isoforms non-covalently associate with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4. CD45 has also been reported to bind galectin-1 and CD22. CD45 isoform expression can change in response to cytokines.

Protocols & Troubleshootings

Related Protocols Staining Cell Surface Targets for Flow Cytometry
Troubleshooting

Troubleshooting for Flow Cytometry

Alternative Formats

Product Name Sizes Price
FITC Anti-Human CD45RO Antibody[UCHL1] 20Tests, 100Tests, 100Tests×2 Check More
PE Anti-Human CD45RO Antibody[UCHL1] 20Tests, 100Tests, 100Tests×2 Check More
APC Anti-Human CD45RO Antibody[UCHL1] 20Tests, 100Tests, 100Tests×2 Check More
PerCP Anti-Human CD45RO Antibody[UCHL1] 20Tests, 100Tests, 100Tests×2 Check More
PE/Cyanine5 Anti-Human CD45RO Antibody[UCHL1] 20Tests, 100Tests, 100Tests×2 Check More
PE/Cyanine7 Anti-Human CD45RO Antibody[UCHL1] 20Tests, 100Tests, 100Tests×2 Check More
PE/Cyanine5.5 Anti-Human CD45RO Antibody[UCHL1] 20Tests, 100Tests, 100Tests×2 Check More
PerCP/Cyanine5.5 Anti-Human CD45RO Antibody[UCHL1] 20Tests, 100Tests, 100Tests×2 Check More
Elab Fluor® 647 Anti-Human CD45RO Antibody[UCHL1] 20Tests, 100Tests, 100Tests×2 Check More
PE/Elab Fluor® 594 Anti-Human CD45RO Antibody[UCHL1] 20Tests, 100Tests, 100Tests×2 Check More

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