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Cat.No.:E-CK-A122D
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Chromosome DNA breakage is an important marker event in cell apoptosis. A series of DNA 3'-OH terminus is produced by DNA double-strand breaks in apoptotic cells or whenever there is a gap in one strand. The fluorescen labeled dUTP can be linked to the 3' -OH terminus of the broken DNA under the action of terminal deoxyribonucleotide transferase (TdT). Fluorescence of dUTP conjugate can be detected by flow cytometer or fluorescence microscope.
Application | DNA Fragmentation |
Detection method | Fluorometric Method |
Sample type | Paraffin section, frozen section, cell slide |
Detection instrument | Fluorescence Microscope |
Dye Type | Elab Fluor® 594 |
Ex/Em (nm) | 590/617 |
Filter set | TRITC |
Storage | -20°C, shading light |
Shipping | Ice bag |
Expiration date | 12 months |