Alternate Names	T-cell surface glycoprotein CD3 epsilon chain,CD3E,T-cell surface antigen T3/Leu-4 epsilon chain,CD3e,CD3E,T3E
Swissprot	P22646
References	1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Davis MM. 1990. Annu. Rev. Biochem. 59:475. 3. Weiss A, et al. 1994. Cell 76:263.

Product Details

Form	Liquid
Clone No.	145-2C11(See Other Available Formats)
Host	Armenian Hamster
Isotype	Armenian Hamster IgG
Isotype Control	Elab Fluor^® 647 Armenian Hamster IgG Isotype Control[PIP] [Product E-AB-F09852M]
Reactivity	Mouse
Application	FCM (See the Application Description)
Storage Buffer	Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use	Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Shipping	Biological ice pack at 4℃
Stability & Storage	Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Conjugation	Elab Fluor^® 647
	Elab Fluor^® 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 670 nm (e.g., a 660/20 nm bandpass filter).

How to select the appropriate detection channel through the spectrogram?

Background

CD3ε is a 20 kD transmembrane protein, also known as CD3 or T3. It is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3ε forms a TCR complex by associating with the CD3δ, γ and ζ chains, as well as the TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex.

Protocols & Troubleshootings

Related Protocols	Staining Cell Surface Targets for Flow Cytometry
Troubleshooting	Troubleshooting for Flow Cytometry

Alternative Formats

Product Name	Sizes	Price
AF/LE Purified Anti-Mouse CD3ε Antibody[145-2C11]	50μg, 500μg, 1mg	Check More
Purified Anti-Mouse CD3ε Antibody[145-2C11]	25μg, 100μg	Check More
Biotin Anti-Mouse CD3ε Antibody[145-2C11]	25μg, 100μg	Check More
FITC Anti-Mouse CD3ε Antibody[145-2C11]	50Tests, 100Tests, 100Tests×2	Check More
PE Anti-Mouse CD3ε Antibody[145-2C11]	50Tests, 100Tests, 100Tests×2	Check More
APC Anti-Mouse CD3ε Antibody[145-2C11]	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5 Anti-Mouse CD3ε Antibody[145-2C11]	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine7 Anti-Mouse CD3ε Antibody[145-2C11]	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5.5 Anti-Mouse CD3ε Antibody[145-2C11]	50Tests, 100Tests, 100Tests×2	Check More
PerCP/Cyanine5.5 Anti-Mouse CD3ε Antibody[145-2C11]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 488 Anti-Mouse CD3ε Antibody[145-2C11]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® Violet 450 Anti-Mouse CD3ε Antibody[145-2C11]	50Tests, 100Tests, 100Tests×2	Check More
FITC Anti-Mouse CD3ε Antibody[145-2C11]	25μg, 100μg	Check More
PE Anti-Mouse CD3ε Antibody[145-2C11]	25μg, 100μg	Check More
APC Anti-Mouse CD3ε Antibody[145-2C11]	25μg, 100μg	Check More
PE/Cyanine5 Anti-Mouse CD3ε Antibody[145-2C11]	25μg, 100μg	Check More
PE/Cyanine7 Anti-Mouse CD3ε Antibody[145-2C11]	25μg, 100μg	Check More
PE/Cyanine5.5 Anti-Mouse CD3ε Antibody[145-2C11]	25μg, 100μg	Check More
PerCP/Cyanine5.5 Anti-Mouse CD3ε Antibody[145-2C11]	25μg, 100μg	Check More
Elab Fluor^® 488 Anti-Mouse CD3ε Antibody[145-2C11]	25μg, 100μg	Check More
Elab Fluor^® 647 Anti-Mouse CD3ε Antibody[145-2C11]	25μg, 100μg	Check More
Elab Fluor^® Violet 450 Anti-Mouse CD3ε Antibody[145-2C11]	25μg, 100μg	Check More

Citations

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