AF (Total Aflatoxin) ELISA Kit

    • Mycotoxin Detection ELISA Kit-Elabscience
    • Mycotoxin Detection ELISA Kit-Elabscience
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    >
    • Mycotoxin Detection ELISA Kit-Elabscience
    • Mycotoxin Detection ELISA Kit-Elabscience
    • Mycotoxin Detection ELISA Kit-Elabscience
    • Mycotoxin Detection ELISA Kit-Elabscience

      Catalog number:E-TO-E006

      Size:
      • 96T
      Qty:
      - +
      Price: $240

      sample_type: Grain,Formul afeed,Edible oil,Peanut,

      Sensitivity: 0.02 ppb

      Lead Time: 7~10 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request

      Test principle


      This kit uses Competitive-ELISA as the method. It can detect Total Aflatoxin (AF) in samples, such as grain, formula feed, edible oil, etc. This kit is composed of ELISA Microtiter plate, HRP conjugate, antibody working solution, standard and other supplementary reagents. The microtiter plate in this kit has been pre-coated with coupled antigen. During the reaction, AF in the samples or standard competes with coupled antigen on the solid phase supporter for sites of anti-AF antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each microtiter plate well, and TMB substrate is added for color development. There is a negative correlation between the OD value of samples and the concentration of AF. The concentration of AF in the samples can be calculated by comparing the OD of the samples to the standard curve.



      Technical indicator

      Sensitivity: 0.02 ppb (ng/mL)

      Reaction mode: 25, 30 min~ 15 min

      Detection limit: Grain---0.1 ppb; Formula feed---0.2 ppb; Edible oil,Peanut---0.2 ppb;

         Biscuit ---0.2 ppb; Beer---0.2 ppb; Wine, Soy sauce, Vinegar---0.1 ppb

      Cross-reactivity: Aflatoxin B1 (AFB1) ---100%, AflatoxinB2 (AFB2) ---80%,

           Aflatoxin G1 (AFG1)--75%, Aflatoxin G2 (AFG2) ---45%,

           Aflatoxin M1 (AFM1) ---8%

       Sample recovery rate: Grain, Formula feed---85%±15%; Peanut---82%±15%;

      Edible oil---85%±15%; Biscuit---83%±15%;

      Beer---84%±15%; Wine, Soy sauce, Vinegar---87%±15%.


      Kits components

      Item

      Specifications

      ELISA Microtiter plate

      96 wells

      Standard Liquid

      1 mL each

      (0 ppb, 0.02 ppb, 0.04 ppb, 0.08 ppb, 0.16 ppb, 0.32 ppb)

      HRP Conjugate

      5.5 mL

      Antibody Working Solution

      5.5 mL

      Substrate Reagent A

      6 mL

      Substrate Reagent B

      6 mL

      Stop Solution

      6 mL

      20×Concentrated Wash Buffer

      40 mL

      Plate Sealer

      3 pieces

      Sealed Bag

      1 piece

      Manual

      1 copy


      Other materials required but not supplied

      Instrument: Microplate reader, Printer, Homogenizer, Nitrogen Evaporators, Water bath, Oscillators, Centrifuge, Graduated pipette, Balance (sensibility 0.01 g).

      High-precision transferpettor: Single channel (20-200 μL, 100-1000 μL), Multichannel (300 μL).

      Reagents: Methanol, N-hexane, Trichloromethane or Dichloromethane.


      Storage and valid period

      Storage:Store at 2-8for 1 year. Avoid freezing.
      Please store the opened kit at 2~8℃, protect from light and moisture. The valid period is 1 months.

      Expiry date: expiration date is on the packing box. 

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