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Alcohol Dehydrogenase (ADH) Activity Assay Kit

  • Cat.No.:E-BC-K763-M

  • Detection instrument: Microplate reader (440-460 nm, optimum wavelength: 450 nm)

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Detection principle

ADH catalyzes the oxidative dehydrogenation of ethanol. Meanwhile, NAD+ is reduced to NADH, Which under the action of hydrogen transmitter, transfer electrons to WST-8 to produce the yellow product. The activity of ADH can be calculated by measuring the change of absorbance value at 450 nm.

Performance characteristics

Synonyms ADH
Sample type serum, plasma, animal tissue
Sensitivity 0.29 U/L
Detection range 0.29-248 U/L
Detection method Colorimetric method
Assay type Enzyme Activity
Assay time 30 min
Precision Average inter-assay CV: 4.500%Average intra-assay CV: 4.200%
Other instruments required Incubator, centrifuge, Microplate reader
Other reagents required Normal saline (0.9% NaCl)
Storage -20℃
Valid period 12 months


ADH activity was significantly changed in each group. (**P<0.01 vs. model group, ##P<0.01 vs. control group)

L Wu et al investigate the hepatoprotective effect of monascin. Alcohol dehydrogenase (ADH) activity of mouse liver was determined using ADH activity assay kit (E-BC-K763-M).


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