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Alcohol Dehydrogenase (ADH) Activity Assay Kit

Cat.No.:E-BC-K763-M
Detection instrument: Microplate reader (440-460 nm, optimum wavelength: 450 nm)

Detection method: Colorimetric method

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Detection principle

ADH catalyzes the oxidative dehydrogenation of ethanol. Meanwhile, NAD+ is reduced to NADH, Which under the action of hydrogen transmitter, transfer electrons to WST-8 to produce the yellow product. The activity of ADH can be calculated by measuring the change of absorbance value at 450 nm.

Performance characteristics

Synonyms	ADH
Sample type	serum, plasma, animal tissue
Sensitivity	0.29 U/L
Detection range	0.29-248 U/L
Detection method	Colorimetric method
Assay type	Enzyme Activity
Assay time	30 min
Precision	Average inter-assay CV: 4.500%Average intra-assay CV: 4.200%
Other instruments required	Incubator, centrifuge, Microplate reader
Other reagents required	Normal saline (0.9% NaCl)
Storage	-20℃
Valid period	12 months

Images

ADH activity was significantly changed in each group. (**P<0.01 vs. model group, ##P<0.01 vs. control group)

L Wu et al investigate the hepatoprotective effect of monascin. Alcohol dehydrogenase (ADH) activity of mouse liver was determined using ADH activity assay kit (E-BC-K763-M).

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

Food Bioscience (2022) IF: 5.318
Ameliorative effects of monascin from red mold rice on alcoholic liver injury and intestinal microbiota dysbiosis in mice

DOI: 10.1016/j.fbio.2022.102079

Sample: livers
Foods (2022) IF: 5.561
Monascuspiloin from Monascus-Fermented Red Mold Rice Alleviates Alcoholic Liver Injury and Modulates Intestinal Microbiota

DOI: 10.3390/foods11193048

PMID: 36230124
Sample: serum

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