ALD(Aldosterone) ELISA Kit

    • competitive-ELISA-Elabscience
    • competitive-ELISA-Elabscience
    • competitive-ELISA-Elabscience
    • competitive-ELISA-Elabscience
    • competitive-ELISA-Elabscience
    • competitive-ELISA-Elabscience
    • competitive-ELISA-Elabscience
    • competitive-ELISA-Elabscience
    • competitive-ELISA-Elabscience

      Catalog number:E-EL-0070


      • 96T
      • 24T
      - +
      Price: $495

      Reactivity: Universal

      Detection Range: 31.25~2000 pg/mL

      Sensitivity: 18.75 pg/mL

      Lead Time: 7~10 daysWelcome to order from local distributors.

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      Intended use

      This ELISA kit applies to the in vitro quantitative determination of  ALD concentrations in serum, plasma and other biological fluids.

      Test principle

      This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with ALD. During the reaction,  ALD in the sample or standard competes with a fixed amount of ALD on the solid phase supporter for sites on the Biotinylated Detection Ab specific to  ALD. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of  ALD in the samples is then determined by comparing the OD of the samples to the standard curve.

      Assay type Competitive
      Format 96T
      Assay time 2.0h
      Reactivity Universal
      Detection Method Colormetric
      Detection Range 31.25—2000 pg/mL
      Sensitivity 18.75 pg/mL
      Sample Volume 50μL
      Sample Type Serum, plasma and other biological fluids


      This kit recognizes ALD in samples. No significant cross-reactivity or interference between ALD and analogues was observed.

      Typical data

      As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

      Concentration(pg/mL) O.D Average
      2000 0.306
      1000 0.42
      500 0.612
      250 0.87
      125 1.259
      62.5 1.647
      31.25 1.949
      0 2.382


      Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level ALD were tested 20 times on one plate, respectively.
      Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level ALD were tested on 3 different plates, 20 replicates in each plate.

        Intra-assay Precision Inter-assay Precision
      Sample 1 2 3 1 2 3
      n 20 20 20 20 20 20
      Mean (pg/mL) 51.80 89.60 476.90 46.80 94.20 479.40
      Standard deviation 2.70 4.30 20.00 3.20 5.70 23.00
      C V (%) 5.21 4.80 4.19 6.84 6.05 4.80


      The recovery of  ALD spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

      Sample Type Range (%) Average Recovery (%)
      Serum (n=5) 86-98 91
      EDTA plasma (n=5) 96-112 102
      Cell culture media (n=5) 90-102 96


      Samples were spiked with high concentrations of ALD and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

          Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5)
      1:2 Range (%) 100-113 91-106 88-101
      Average (%) 105 98 95
      1:4 Range (%) 94-107 87-103 87-98
      Average (%) 101 95 92
      1:8 Range (%) 101-117 95-106 85-98
      Average (%) 107 100 91
      1:16 Range (%) 97-109 87-101 89-99
      Average (%) 104 94 94

      Kit components & Storage

      An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

      Item Specifications Storage
      Micro ELISA Plate(Dismountable) 8 wells ×12 strips -20℃, 6 months
      Reference Standard 2 vials
      Concentrated Biotinylated Detection Ab (100×) 1 vial, 120 μL
      Concentrated HRP Conjugate (100×) 1 vial, 120 μL -20℃(shading light), 6 months
      Reference Standard & Sample Diluent 1 vial, 20 mL 2-8℃, 6 months
      Biotinylated Detection Ab Diluent 1 vial, 14 mL
      HRP Conjugate Diluent 1 vial, 14 mL
      Concentrated Wash Buffer (25×) 1 vial, 30 mL
      Substrate Reagent 1 vial, 10 mL 2-8℃(shading light)
      Stop Solution 1 vial, 10 mL 2-8℃
      Plate Sealer 5 pieces
      Product Description 1 copy
      Certificate of Analysis 1 copy

      Note: All reagent bottle caps must be tightened to prevent evaporation and microbial pollution.
      The volume of reagents in partial shipments is a little more than the volume marked on the label, please use accurate measuring equipment instead of directly pouring into the vial(s).

      Other supplies required

      • Microplate reader with 450 nm wavelength filter
      • High-precision transfer pipette, EP tubes and disposable pipette tips
      • Incubator capable of maintaining 37℃
      • Deionized or distilled water
      • Absorbent paper
      • Loading slot for Wash Buffer

      Assay Procedure

      elisa assay procedure 1

      1.Add 50 μL standard or sample to each well.

      elisa assay procedure 2

      2.Immediately add 50 μL Biotinylated Detection Ab to each well.

      elisa assay procedure 3

      3.Incubate for 45 min at 37℃. Aspirate and wash 3 times.

      elisa assay procedure 4

      4.Add 100 μL HRP Conjugate to each well. Incubate for 30 min at 37℃. Aspirate and wash 5 times.

      elisa assay procedure 5

      5.Add 90 μL Substrate Reagent. Incubate for 15 min at 37℃.

      elisa assay procedure 6

      6.Add 50 μL Stop Solution.

      elisa assay procedure 7

      7.Read at 450 nm immediately. Calculation of results.


      1. Publication: Luo X, Wang D, Luo X, et al. Caveolin 1-Related Autophagy Initiated By Aldosterone-Induced Oxidation Promotes Liver Sinusoidal Endothelial Cells Defenestration[J]. Redox Biology, 2017.
        Species: Universal
        Sample Type: Serum
      2. Publication: Yuan P P, Zheng X K, Li M, et al. Two Sulfur Glycoside Compounds Isolated From Lepidium Apetalum Willd Protect NRK52e Cells Against Hypertonic-Induced Adhesion And Inflammation By Suppressing The MAPK Signaling Pathway And RAAS[J]. Molecules, 2017.
        Species: Rat, Universal
        Sample Type: Culture supernatant
      3. Publication: Huo R, Zeng B, Zeng L, et al. Microbiota Modulate Anxiety-Like Behavior And Endocrine Abnormalities In Hypothalamic-Pituitary-Adrenal Axis[J]. Frontiers In Cellular And Infection Microbiology, 2017.
        Species: Mouse, Universal
        Sample Type: Tissue homogenate
      4. Publication: Siboto A, Sibiya N, Khathi A, et al. The Effects of Momordica balsamina Methanolic Extract on Kidney Function in STZ-Induced Diabetic Rats: Effects on Selected Metabolic Markers[J]. Journal of Diabetes Research, 2018, 2018.
        Species: Rat
      5. Publication: Alfred M G, Tagumirwa M C. Transdermal Drug Delivery of Asiatic Acid Influences Renal Function and Electrolyte Handling in Plasmodium berghei-Infected Sprague-Dawley Male Rats[J]. Journal of Diseases and Medicinal Plants, 2018, 4(1): 18-29.
        Species: Human
        Sample Type: Blood
      6. Publication: Zeng M, Zhang L, Li M, et al. Mechanism of the diuretic activity of Descurainia sophia seed[J]. Bangladesh Journal of Pharmacology, 13(2): 157-163, May 15, 2018.
        Species: Rat
        Sample Type: Urine
      7. Publication: Yuan P , Zheng X , Li M , et al. Two Sulfur Glycoside Compounds Isolated from Lepidium apetalum Willd Protect NRK52e Cells against Hypertonic-Induced Adhesion and Inflammation by Suppressing the MAPK Signaling Pathway and RAAS[J]. Molecules, 2017, 22(11):1956.
        Species: Rat
        Sample Type: Cell culture supernatant
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