Am (Amantadine) ELISA Kit

    • Food Safety ELISA Kit-Elabscience
    • Food Safety ELISA Kit-Elabscience
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    >
    • Food Safety ELISA Kit-Elabscience
    • Food Safety ELISA Kit-Elabscience
    • Food Safety ELISA Kit-Elabscience
    • Food Safety ELISA Kit-Elabscience

      Catalog number:E-FS-E085

      Size:
      • 96T
      Qty:
      - +
      Price: $370

      sample_type: Tissue,Eggss,Milk

      Sensitivity: 0.5 ppb

      Lead Time: 7~10 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request Manual

      Test principle

      This kit uses Indirect-Competitive-ELISA as the method. It can be used to detect Amantadine in samples, such as tissues, eggs, milk etc. This kit is composed of ELISA Microtiter plate, HRP conjugate, antibody, standard and other supplementary reagents. The micro-plate provided in this kit has been pre-coated with Amantadine antigen. During the reaction, Amantadine in the samples or standard competes with Amantadine antigen on the solid phase supporter for sites of Amantadine antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each micro plate well, and TMB substrate is added for color development. There is a negative correlation between the OD value of samples and the concentration of Amantadine. The concentration of Amantadine in the samples can be calculated by comparing the OD of the samples to the standard curve.


      Technical indicator

      Sensitivity: 0.5 ppb (ng/mL)

      Reaction mode: 25℃, 30 min~20 min

      Detection limit: Tissue, Eggs ---1 ppb, Milk---2 ppb. 

      Cross-reactivity:

      Amantadine

      100%

      Rimantadine

      230%

      Somantadine

      240%

      Memantine

      1%

      Sample recovery rate: Eggs, Tissue, Milk ---90%±30%.


      Kits components

      Item

      Specifications

      ELISA Microtiter plate

      96 wells

      Standard Liquid

      1.5 mL each

            (0 ppb, 0.5 ppb, 1.5 ppb, 4.5 ppb, 13.5 ppb, 40.5 ppb)

      Antibody working Solution

      7 mL

      HRP Conjugate

      7 mL

      20×Concentrated Sample Solution

      10 mL

      Substrate Reagent A

      7 mL

      Substrate Reagent B

      7 mL

      Stop Solution

      7 mL

      20×Concentrated Wash Buffer

      25 mL

      Plate Sealer

      3 pieces

      Sealed Bag

      1 piece

      Manual

      1 copy



      Other supplies required

      Instrument: Microplate reader, Printer, Homogenizer, Nitrogen Evaporators/Water bath, Oscillators, Centrifuge, Graduated pipette, Balance (sensibility 0.01g),

      High-precision transferpettor: Single-channel (20-200 μL, 100-1000 μL), Multi-channel (300 μL) 

      Reagents: Acetonitrile (analytical reagent), NaCl (analytical reagent), ZnSO4.7H2O.



      Storage and valid period

      Store at 2~8℃ for 1 year. Avoid freeze.

      Please store the opened kit at 2~8℃, protect from light and moisture. The valid period is 1 months.

      Expiry date: expiration date is on the packing box.



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