Annexin V Binding Buffer(10×)[151]

    • Annexin V Binding Buffer(10 ×)-Elabscience
    • Annexin V Binding Buffer(10 ×)-Elabscience
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    • Annexin V Binding Buffer(10 ×)-Elabscience
    • Annexin V Binding Buffer(10 ×)-Elabscience
    • Annexin V Binding Buffer(10 ×)-Elabscience
    • Annexin V Binding Buffer(10 ×)-Elabscience

      Catalog number:E-CK-A151

      Size:
      • 10mL
      • 50mL
      Qty:
      - +
      Price: $60

      Lead Time: 7~10 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request Manual

      Introduction:

      Elabscience® Annexin V Binding Buffer (10 ×) is developed to identify apoptotic and necrotic cells.


      Components:


      Cat.

      Products

      10 mL

      50 mL

      Storage

       E-CK-A151

       Annexin V Binding Buffer(10 ×)

      10 mL

      50 mL

      2-8

                                                 Manual

       One Copy



      Self-Prepared Reagent:

      DI water


      Instructions:

      The Annexin V Binding Buffer (10 ×)[Cat:E-CK-A151] is a 10 × concentrated solution. Dilute with DI water to 1 × working solution before use.

      For example: Take 1 mL Annexin V Binding Buffer (10 ×), dilute with DI water to 10 mL.


      Staining Procedure:

      1. Induce apoptosis of suspension cells with reagents of interest. Collect cell cultures and centrifuge at 300 g for 5 min, discard the supernatant. Add appropriate PBS to wash the cells, resuspend gently and count the cells.

      Note: This product is only validated in suspension cells. Good cell viability is the key to the experiment. When the adherent cells are used for apoptotic detection, treatments like digestion may increase the ratio of necrotic or apoptotic cells and cause uncontrollable effects on the experimental results. Please be aware!

      2. Split the cell suspension into tubes, 1-5 × 105 cells for each. Centrifuge at 300 g for 5 min, discard the supernatant. Add appropriate PBS to wash the cells and discard the supernatant. Add 500 μL of 1 × Annexin V Binding Buffer to resuspend the cells.

      3. Add 5 µl of Annexin V- fluorescein and 5 µl of DNA dye (PI[#Cat:E-CK-A161] or 7-AAD[#Cat:E-CK-A162]) to each tube.

      4. Gently vortex the cells and incubate at room temperature for 15-20 min in the dark.

      5. Analyze the cells immediately with proper machine settings. Otherwise, place the cells on ice in the dark and analyze within 1 h.


      Storage:

      Store at 4°C for up to one year.

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