Annexin V-Cyanine5 / PI Apoptosis Detection Kit[221]


      Catalog number:E-CK-A221

      • 20T
      • 50T
      • 100T
      • 200T
      - +
      Price: $75

      Applications: Apoptosis

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      Elabscience® Annexin V-Cyanine5 / PI Cell Apoptosis Detection kit is developed to identify apoptotic and necrotic cells.

      Annexin V is a member of the annexin family, which binds to phosphatidylserine (PS) in a calcium-dependent manner. Annexin V-Cyanine5, the Cyanine5-conjugated format, binds specifically to the PS on the outer leaflet of apoptotic cell membrane and can be detected with flow cytometry or fluorescence microscopy.

      Propidium Iodide (PI) is a common DNA dye that is not permeable to cell membrane. Once binding to DNA, the flurescence of PI increases by nearly 20 fold. Due to the loss of integrity of membrane, PI can enter late apoptotic or necrotic cells to stain DNA. Cells at different apoptotic stages can be distinguished by using Annexin V and PI.




      20 Tests

      50 Tests

      100 Tests

      200 Tests



      Annexin V- Cyanine5 Reagent

      100 μL

      250 μL

      500 μL

      1 mL



      Annexin V Binding Buffer (10 ×)

      1.4 mL×2

      5.5 mL

      11 mL

      11 mL×2



      Propidium Iodide (PI) Staining Solution

      100 μL

      250 μL

      500 μL

      1 mL



       One Copy

      Jurkat cells were treated with 5 μM Camptothecin and detected with this kit.

      Jurkat cells were cultured with (Right) or without (Left) 5 μM Camptothecin for 4 h. Annexin V-Cyanine5 single-positive cells were early apoptotic cells, Annexin V-Cyanine5 and PI double-positive cells were necrotic or late apoptotic cells, and PI single-positive cells were naked nuclei.


      The Annexin V Binding Buffer (10 ×)[E-CK-A151] is a 10 × concentrated solution. Dilute with DI water to 1 × working solution before use.

      For example: Take 1 mL Annexin V Binding Buffer (10 ×), dilute with DI water to 10 mL.


      Store at 2~8°C for one year. Annexin V-Cyanine5 and PI Staining Solution should be stored in dark.


      1. For maximal assay performance, this kit should be used within 12 months. Avoid freeze / thaw cycles.

      2. For FCM analysis, please set untreated cells stained with both Annexin V-Cyanine5 and PI as negative control. As for compensation controls, please use drug-treated cells stained with either Annexin V-Cyanine5 or PI.

      3. Annexin V-Cyanine5 can be detected in APC channel while PerCP/Cy5.5 channel is preferred to PE channel for PI detection due to the large compensation needed between APC and PE channels.

      4. Detect apoptosis as soon as possible after staining to avoid the increase number of apoptosis or necrosis

      5. Avoid extended exposure of the samples to direct light to protect the fluorophores from quenching.

      6. For your safety and health, please wear the lab coat and disposable gloves before the experiments.

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