Annexin V-FITC / PI Apoptosis Detection Kit[211]

    • Annexin V-FITC / PI Apoptosis Detection Kit-Elabscience
    • Annexin V-FITC / PI Apoptosis Detection Kit-Elabscience
    • Annexin V-FITC / PI Apoptosis Detection Kit-Elabscience
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    >
    • Annexin V-FITC / PI Apoptosis Detection Kit-Elabscience
    • Annexin V-FITC / PI Apoptosis Detection Kit-Elabscience
    • Annexin V-FITC / PI Apoptosis Detection Kit-Elabscience
    • Annexin V-FITC / PI Apoptosis Detection Kit-Elabscience
    • Annexin V-FITC / PI Apoptosis Detection Kit-Elabscience
    • Annexin V-FITC / PI Apoptosis Detection Kit-Elabscience

      Catalog number:E-CK-A211

      Size:
      • 20T
      • 50T
      • 100T
      • 200T
      Qty:
      - +
      Price: $75

      Applications: Apoptosis

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      Introduction

      Elabscience® Annexin V-FITC/PI Cell Apoptosis Detection kit is developed to identify apoptotic and necrotic cells.

      Annexin V is a member of the annexin family, which binds to phosphatidylserine (PS) in a calcium-dependent manner. Annexin V-FITC, the FITC-conjugated format, binds specifically to the PS on the outer leaflet of apoptotic cell membrane and can be detected with flow cytometry or fluorescence microscopy.

      Propidium Iodide (PI) is a common DNA dye that is not permeable to cell membrane. Once binding to DNA, the flurescence of PI increases by nearly 20 fold. Due to the loss of integrity of membrane, PI can enter late apoptotic or necrotic cells to stain DNA. Cells at different apoptotic stages can be distinguished by using Annexin V and PI.



      Components

      Cat.

      Products

      20 Tests

      50 Tests

      100 Tests

      200 Tests

      Storage

      E-CK-A111

      Annexin V-FITC Reagent

      100 μL

      250 μL

      500 μL

      1 mL

      2~8°C

      E-CK-A151

      Annexin V Binding Buffer (10 ×)

      1.4 mL×2

      5.5 mL

      11 mL

      11 mL×2

      2~8°C

      E-CK-A161

      Propidium Iodide (PI) Staining Solution

      100 μL

      250 μL

      500 μL

      1 mL

      2~8°C

      Manual

       One Copy



      Jurkat cells were treated with 5 μM Camptothecin and detected with this kit.


      Jurkat cells were cultured with (Left) or without (Right) 5 μM Camptothecin for 4 h. Annexin V-FITC single-positive cells were early apoptotic cells, Annexin V-FITC and PI double-positive cells were necrotic or late apoptotic cells, and PI single-positive cells were naked nuclei.


      Instructions

      The Annexin V Binding Buffer (10 ×)[E-CK-A151] is a 10 × concentrated solution. Dilute with DI water to 1 × working solution before use.

      For example: Take 1 mL Annexin V Binding Buffer (10 ×), dilute with DI water to 10 mL.


      Storage

      Store at 2~8°C. Annexin V-FITC and Propidium Iodide (PI)  Staining Solution should be stored in dark.


      Cautions

      1. For maximal assay performance, this kit should be used within 12 months. Avoid freeze / thaw cycles.

      2. For FCM analysis, please set untreated cells stained with both Annexin V-FITC and PI as negative control. As for compensation controls, please use drug-treated cells stained with either Annexin V-FITC or PI.

      3. Annexin V-FITC can be detected in FITC channel while PerCP/Cy5.5 channel is preferred to PE channel for PI detection due to the large compensation needed between FITC and PE channels.

      4. Detect apoptosis as soon as possible after staining to avoid the increase number of apoptosis or necrosis.

      5. Avoid extended exposure of the samples to direct light to protect the fluorophores from quenching.

      6. For your safety and health, please wear the lab coat and disposable gloves before the experiments.

      Citations

      1. Demethoxycurcumin analogue DMC-BH inhibits orthotopic growth of glioma stem cells by targeting JNK/ERK signaling
        IF: 4.831
        Journal: Aging (Albany NY)(2020)
        DOI: 10.18632/aging.103531
        PMID: 32710727
        Products: E-CK-A211 
        Sample type: Cell sample
        Reactivity: Human
      2. Human Antimicrobial Peptide Hepcidin 25-Induced Apoptosis in Candida albicans
        IF: 4.152
        Journal: Microorganisms(2020)
        DOI: 10.3390/microorganisms8040585
        PMID: 32316661
        Products: E-CK-A211 
        Sample type: Cell sample
        Reactivity: Bacteria
      3. Anticancer e ects of brusatol in nasopharyngeal carcinoma through suppression of the Akt/mTOR signaling pathway
        IF: 3.008
        Journal: Cancer Chemotherapy and Pharmacology(2020)
        DOI: 10.1007/s00280-020-04083-3
        PMID: 99999999
        Products: E-CK-A211 
        Sample type: Cell lysate
        Reactivity: Human
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      Verified Customer

      w**tSubmitted [ Dec 04 2019 ]

      • Application:FCM
      • Description:i like this produce, because the separation of apoptotic cells from normal cells is good. I get results very well
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      MSDS for ELISA ISO 9001

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