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APC Anti-Human CD117/c-Kit Antibody[104D2]

Uniprot : P10721
  • Cat.No.:E-AB-F1150E

  • Applications: FCM
  • Isotype: Mouse IgG1, κ
  • Host: Mouse

To Purchase E-AB-F1150E

Size:
  • 20Tests
  • 100Tests
  • 100Tests×2
Price: $90
Qty:

Antigen Details

Alternate Names Mast/stem cell growth factor receptor Kit,Kit,SCFR,Proto-oncogene c-Kit,Tyrosine-protein kinase Kit,CD117
Swissprot P10721
Gene ID 3815
References
1. Giebel LB, et al. 1992. Oncogene 7:2207.
2. Furitsu T, et al. 1993. J. Clin. Invest. 92:1736.

Product Details

Form Liquid
Clone No. 104D2(See Other Available Formats)
Host Mouse
Isotype Mouse IgG1, κ
Isotype Control APC Mouse IgG1, κ Isotype Control[MOPC-21] [Product E-AB-F09792E]
Reactivity Human
Application FCM (See the Application Description)
Storage Buffer Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Shipping Biological ice pack at 4℃
Stability & Storage Keep as concentrated solution.
Store at 2~8°C and protected from prolonged exposure to light. Do not freeze.
Centrifuge before opening to ensure complete recovery of vial contents.
This product is guaranteed up to one year from purchase.
Conjugation APC
APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter).


How to select the appropriate detection channel through the spectrogram?

Background

CD117 is a 145 kD protein tyrosine kinase also known as c-Kit. It is a receptor for stem cell factor or c-Kit ligand. CD117 is expressed on pluripotent hematopoietic progenitor cells (approximately 1-4% bone marrow cells), mast cells, and acute myeloid leukemia cells (AML). CD117 binding of c-Kit ligand induces phosphorylation of CD117 and stimulates proliferation and survival of primitive hematopoietic stem cells as well as erythroid-committed and granulo-monocytic committed cells.

Protocols & Troubleshootings

Related Protocols Staining Cell Surface Targets for Flow Cytometry
Troubleshooting

Troubleshooting for Flow Cytometry

Alternative Formats

Product Name Sizes Price
FITC Anti-Human CD117/c-Kit Antibody[104D2] 20Tests, 100Tests, 100Tests×2 Check More
PE Anti-Human CD117/c-Kit Antibody[104D2] 20Tests, 100Tests, 100Tests×2 Check More
PE/Cyanine7 Anti-Human CD117/c-Kit Antibody[104D2] 20Tests, 100Tests, 100Tests×2 Check More

Citations

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