Alternate Names	B7-H1, PD-L1,Programmed cell death ligand 1,B7 homolog 1,B7-H, B7H1, PDL1, PDCD1L1, PDCD1LG1
Swissprot	Q9NZQ7
References	1. Sharpe A, et al. 2007. Nat. Immunol. 8:239.

Product Details

Form	Liquid
Clone No.	29E.2A3(See Other Available Formats)
Host	Mouse
Isotype	Mouse IgG2b, κ
Isotype Control	APC Mouse IgG2b, κ Isotype Control[MPC-11] [Product E-AB-F09812E]
Reactivity	Human
Application	FCM (See the Application Description)
Storage Buffer	Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use	Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Shipping	Biological ice pack at 4℃
Stability & Storage	Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Conjugation	APC
	APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter).

How to select the appropriate detection channel through the spectrogram?

Background

CD274, also known as PD-L1 and B7-H1, is type I transmembrane glycoprotein that serves as a ligand for CD279 (PD-1). This interaction is believed to regulate the balance between the stimulatory and inhibitory signals needed for responses to microbes and maintenance of self-tolerance. CD274 is involved in the costimulation of T cell proliferation and IL-10 and IFN-γ production in an IL-2-dependent and CD279-independent manner. Conflicting data has shown that CD274 can inhibit T cell proliferation and cytokine production, and alternatively, enhance T cell activation. Other studies suggest that CD274 may signal bidirectionally, raising interesting implications for its expression in a wide variety of cell types, including T and B cells, antigen-presenting cells, and nonhematopoietic cells.

Protocols & Troubleshootings

Related Protocols	Staining Cell Surface Targets for Flow Cytometry
Troubleshooting	Troubleshooting for Flow Cytometry

Alternative Formats

Product Name	Sizes	Price
AF/LE Purified Anti-Human CD274/PD-L1 Antibody[29E.2A3]	50μg, 500μg, 1mg	Check More
Purified Anti-Human CD274/PD-L1 Antibody[29E.2A3]	25μg, 100μg	Check More
Biotin Anti-Human CD274/PD-L1 Antibody[29E.2A3]	25μg, 100μg	Check More
FITC Anti-Human CD274/PD-L1 Antibody[29E.2A3]	20Tests, 100Tests, 100Tests×2	Check More
PE Anti-Human CD274/PD-L1 Antibody[29E.2A3]	20Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5 Anti-Human CD274/PD-L1 Antibody[29E.2A3]	20Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine7 Anti-Human CD274/PD-L1 Antibody[29E.2A3]	20Tests, 100Tests, 100Tests×2	Check More
PerCP/Cyanine5.5 Anti-Human CD274/PD-L1 Antibody[29E.2A3]	20Tests, 100Tests, 100Tests×2	Check More

Citations

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