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APC Anti-Mouse CD117/c-Kit Antibody[2B8]

Uniprot : P05532
  • Cat.No.:E-AB-F1092UE

  • Applications: FCM
  • Isotype: Rat IgG2b, κ
  • Host: Rat

To Purchase E-AB-F1092UE

Size:
  • 25μg
  • 100μg
Price: $72
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Antigen Details

Alternate Names Mast/stem cell growth factor receptor Kit,Kit,SCFR,Proto-oncogene c-Kit,Tyrosine-protein kinase Kit,CD117
Swissprot P05532
References
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Galli SJ, et al. 1994. Adv. Immunol. 55:1.
3. Ikuta K, et al. 1992. Annu. Rev. Immunol. 10:759.
4. Besmer P, et al. 1986. Nature 320:415.
5. Witte ON. 1990. Cell 63:5.

Product Details

Form Liquid
Clone No. 2B8(See Other Available Formats)
Host Rat
Isotype Rat IgG2b, κ
Isotype Control APC Rat IgG2b, κ Isotype Control[LTF-2] [Product E-AB-F09843E]
Reactivity Mouse
Application FCM (See the Application Description)
Storage Buffer Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/106 cells in 100 μL volume].
Shipping Biological ice pack at 4℃
Stability & Storage Keep as concentrated solution.
Store at 2~8°C and protected from prolonged exposure to light. Do not freeze.
Centrifuge before opening to ensure complete recovery of vial contents.
This product is guaranteed up to one year from purchase.
Conjugation APC
APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter).


How to select the appropriate detection channel through the spectrogram?

Background

CD117 is a 145 kD immunoglobulin superfamily member also known as c-Kit and stem cell factor receptor (SCFR). It is a transmembrane tyrosine-kinase receptor that binds the c-Kit ligand (also known as steel factor, stem cell factor, and mast cell growth factor). CD117 is expressed on hematopoietic stem cells (including multipotent hematopoietic stem cells, progenitors committed to myeloid and/or erythroid lineages, and T and B cell precursors), mast cells, and acute myeloid leukemia (AML) cells. CD117 interaction with its ligand is critical for the development of hematopoietic stem cells.

Protocols & Troubleshootings

Related Protocols Staining Cell Surface Targets for Flow Cytometry
Troubleshooting

Troubleshooting for Flow Cytometry

Citations

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