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APC Anti-Mouse CD122/IL-2RB Antibody[5H4]

Uniprot : P16297
  • Cat.No.:E-AB-F1029E

  • Applications: FCM
  • Isotype: Rat IgG2a, κ
  • Host: Rat

To Purchase E-AB-F1029E

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  • 100Tests×2
Price: $40
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Antigen Details

Alternate Names Interleukin-2 receptor subunit beta,Il2rb,IL-2 receptor subunit beta, IL-2R subunit beta,IL-2RB,High affinity IL-2 receptor subunit beta,p70-75,CD122
Swissprot P16297
Gene ID 16185
References
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Minami Y, et al. 1993. Annu. Rev. Immunol. 11:245.
3. Suzuki H, et al. 1995. Science 268:1472.
4. Shi Z, et al. 2009. Eur. J. Immunol. 39:2109.

Product Details

Form Liquid
Clone No. 5H4(See Other Available Formats)
Host Rat
Isotype Rat IgG2a, κ
Isotype Control APC Rat IgG2a, κ Isotype Control[2A3] [Product E-AB-F09832E]
Reactivity Mouse
Application FCM (See the Application Description)
Storage Buffer Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Shipping Biological ice pack at 4℃
Stability & Storage Keep as concentrated solution.
Store at 2~8°C and protected from prolonged exposure to light. Do not freeze.
Centrifuge before opening to ensure complete recovery of vial contents.
This product is guaranteed up to one year from purchase.
Conjugation APC
APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter).


How to select the appropriate detection channel through the spectrogram?

Background

CD122 is a 70-75 kD IL-2 receptor β chain also known as IL-2Rβ, which is also shared by the IL-15 receptor. It is constitutively expressed by NK cells and at lower levels by T cells, B cells, monocytes, and macrophages. The IL-2Rβ chain can combine with either the common γ subunit (γc, CD132) alone or with the γc subunit and the IL-2Rα subunit (CD25) to generate intermediate or high affinity IL-2 receptor complexes, respectively. CD122 expression levels can be upregulated by activation. The 5H4 antibody does not block IL-2 binding to the IL-2 receptor. CD122 is expressed on murine, but not human, CD8+ Tregs involved in the maintenance of T cell homeostasis.

Protocols & Troubleshootings

Related Protocols Staining Cell Surface Targets for Flow Cytometry
Troubleshooting

Troubleshooting for Flow Cytometry

Alternative Formats

Product Name Sizes Price
AF/LE Purified Anti-Mouse CD122/IL-2RB Antibody[5H4] 50μg, 500μg, 1mg Check More
Purified Anti-Mouse CD122/IL-2RB Antibody[5H4] 25μg, 100μg Check More
Biotin Anti-Mouse CD122/IL-2RB Antibody[5H4] 25μg, 100μg Check More
FITC Anti-Mouse CD122/IL-2RB Antibody[5H4] 50Tests, 100Tests, 100Tests×2 Check More
PE Anti-Mouse CD122/IL-2RB Antibody[5H4] 50Tests, 100Tests, 100Tests×2 Check More
PE/Cyanine5 Anti-Mouse CD122/IL-2RB Antibody[5H4] 50Tests, 100Tests, 100Tests×2 Check More
PE/Cyanine7 Anti-Mouse CD122/IL-2RB Antibody[5H4] 50Tests, 100Tests, 100Tests×2 Check More
PE/Cyanine5.5 Anti-Mouse CD122/IL-2RB Antibody[5H4] 50Tests, 100Tests, 100Tests×2 Check More
Elab Fluor® 488 Anti-Mouse CD122/IL-2RB Antibody[5H4] 50Tests, 100Tests, 100Tests×2 Check More
Elab Fluor® 647 Anti-Mouse CD122/IL-2RB Antibody[5H4] 50Tests, 100Tests, 100Tests×2 Check More
FITC Anti-Mouse CD122/IL-2RB Antibody[5H4] 25μg, 100μg Check More
PE Anti-Mouse CD122/IL-2RB Antibody[5H4] 25μg, 100μg Check More
APC Anti-Mouse CD122/IL-2RB Antibody[5H4] 25μg, 100μg Check More
PE/Cyanine5 Anti-Mouse CD122/IL-2RB Antibody[5H4] 25μg, 100μg Check More
PE/Cyanine7 Anti-Mouse CD122/IL-2RB Antibody[5H4] 25μg, 100μg Check More
PE/Cyanine5.5 Anti-Mouse CD122/IL-2RB Antibody[5H4] 25μg, 100μg Check More
Elab Fluor® 488 Anti-Mouse CD122/IL-2RB Antibody[5H4] 25μg, 100μg Check More
Elab Fluor® 647 Anti-Mouse CD122/IL-2RB Antibody[5H4] 25μg, 100μg Check More

Citations

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