Alternate Names	P-selectin glycoprotein ligand 1,Selplg,PSGL-1,Selectin P ligand,CD162
Swissprot	Q62170
References	1. Borges E, Eytner R, Moll T, et al.Blood. 1997; 90(5):1934-1942. 2. Borges E, Tietz W, Steegmaier M, et al. J Exp Med. 1997; 185(3):573-578. 3. Frenette PS, Denis CV, Weiss L, et al. . J Exp Med. 2000; 191(8):1413-1422.

Product Details

Form	Liquid
Clone No.	4RA10(See Other Available Formats)
Host	Rat
Isotype	Rat IgG1, κ
Isotype Control	APC Rat IgG1, κ Isotype Control[HRPN] [Product E-AB-F09822E]
Reactivity	Mouse
Application	FCM (See the Application Description)
Storage Buffer	Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use	Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Shipping	Biological ice pack at 4℃
Stability & Storage	Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Conjugation	APC
	APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter).

How to select the appropriate detection channel through the spectrogram?

Background

The 4RA10 antibody reacts with the N-terminal functional peptide of CD162 (P-selectin glycoprotein ligand-1, PSGL-1), encoded by the Selpl gene. PSGL-1 is expressed on the cell surface as a homodimer of approximately 230 kDa. In the mouse, Selpl mRNA is detected in most tissues, with high levels found in hematopoietic cells, brain, and adipose tissue. Flow cytometric analyses have revealed CD162 expression on bone marrow-derived mast and dendritic cells, splenic leukocytes, platelets, peripheral blood neutrophils, and neutrophil and T-cell lines. PSGL-1 is a ligand for P-selectin (CD62P) and is involved in leukocyte rolling, the migration of leukocytes into inflamed tissues, and responses to vascular injury. It is a sialomucin that must be specifically sialylated, fucosylated, and sulfated to bind P-selectin. There is also evidence that other ligands for PSGL-1 and CD62P may exist. 4RA10 mAb is reported to block the binding of mouse leukocytes to CD62P and CD62L.

Protocols & Troubleshootings

Related Protocols	Staining Cell Surface Targets for Flow Cytometry
Troubleshooting	Troubleshooting for Flow Cytometry

Alternative Formats

Product Name	Sizes	Price
AF/LE Purified Anti-Mouse CD162 Antibody[4RA10]	50μg, 500μg, 1mg	Check More
Purified Anti-Mouse CD162 Antibody[4RA10]	25μg, 100μg	Check More
Biotin Anti-Mouse CD162 Antibody[4RA10]	25μg, 100μg	Check More
FITC Anti-Mouse CD162 Antibody[4RA10]	50Tests, 100Tests, 100Tests×2	Check More
PE Anti-Mouse CD162 Antibody[4RA10]	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5 Anti-Mouse CD162 Antibody[4RA10]	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine7 Anti-Mouse CD162 Antibody[4RA10]	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5.5 Anti-Mouse CD162 Antibody[4RA10]	50Tests, 100Tests, 100Tests×2	Check More
PerCP/Cyanine5.5 Anti-Mouse CD162 Antibody[4RA10]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 488 Anti-Mouse CD162 Antibody[4RA10]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 647 Anti-Mouse CD162 Antibody[4RA10]	50Tests, 100Tests, 100Tests×2	Check More
PE/Elab Fluor^® 594 Anti-Mouse CD162 Antibody[4RA10]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® Violet 450 Anti-Mouse CD162 Antibody[4RA10]	50Tests, 100Tests, 100Tests×2	Check More
FITC Anti-Mouse CD162 Antibody[4RA10]	25μg, 100μg	Check More
PE Anti-Mouse CD162 Antibody[4RA10]	25μg, 100μg	Check More
APC Anti-Mouse CD162 Antibody[4RA10]	25μg, 100μg	Check More
PE/Cyanine5 Anti-Mouse CD162 Antibody[4RA10]	25μg, 100μg	Check More
PE/Cyanine7 Anti-Mouse CD162 Antibody[4RA10]	25μg, 100μg	Check More
PE/Cyanine5.5 Anti-Mouse CD162 Antibody[4RA10]	25μg, 100μg	Check More
PerCP/Cyanine5.5 Anti-Mouse CD162 Antibody[4RA10]	25μg, 100μg	Check More
Elab Fluor^® 488 Anti-Mouse CD162 Antibody[4RA10]	25μg, 100μg	Check More
Elab Fluor^® 647 Anti-Mouse CD162 Antibody[4RA10]	25μg, 100μg	Check More
PE/Elab Fluor^® 594 Anti-Mouse CD162 Antibody[4RA10]	25μg, 100μg	Check More
Elab Fluor^® Violet 450 Anti-Mouse CD162 Antibody[4RA10]	25μg, 100μg	Check More

Citations

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