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Cat.No.:E-AB-F1192UE
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Alternate Names | TIM3, TIMD3,HAVcr-2,TIMD-3 |
Swissprot | Q8VIM0 |
References |
1. Hafler DA and Kuchroo V. 2008. J. Exp. Med. 205:2699.
2. Zhu C, et al. 2005. Nat. Immunol. 6:1245. 3. Wang F, et al. 2009. Immunobiology 214:342. |
Form | Liquid |
Clone No. | RMT3-23(See Other Available Formats) |
Host | Rat |
Isotype | Rat IgG2a, κ |
Isotype Control | APC Rat IgG2a, κ Isotype Control[2A3] [Product E-AB-F09833E] |
Reactivity | Mouse |
Application | FCM (See the Application Description) |
Storage Buffer | Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant. |
Recommended Use | Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/106 cells in 100 μL volume]. |
Shipping | Biological ice pack at 4℃ |
Stability & Storage | Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase. |
Conjugation | APC |
APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter). | |
How to select the appropriate detection channel through the spectrogram?
CD366 (Tim-3) is a transmembrane protein also known as T cell immunoglobulin and mucin domain containing protein-3. Tim-3 is expressed at high levels on activated T cells (preferentially on Th1 cells, monocytes/macrophages, and dendritic cells). Tim-3 has also been shown to exist as a soluble protein. Cells expressing Tim-3 are present at high levels in the CNS of animals at the onset of experimental autoimmune encephalomyelitis (EAE), a disease mediated by lymphocytes secreting Th1-like cytokines. Tim-3 has been proposed to inhibit Th1-mediated immune responses and promote immunological tolerance. |
Related Protocols | Staining Cell Surface Targets for Flow Cytometry |
Troubleshooting |
Product Name | Sizes | Price |
AF/LE Purified Anti-Mouse CD366/Tim-3 Antibody[RMT3-23] | 50μg, 500μg, 1mg | Check More |
Purified Anti-Mouse CD366/Tim-3 Antibody[RMT3-23] | 25μg, 100μg | Check More |
PE Anti-Mouse CD366/Tim-3 Antibody[RMT3-23] | 50Tests, 100Tests, 100Tests×2 | Check More |
APC Anti-Mouse CD366/Tim-3 Antibody[RMT3-23] | 50Tests, 100Tests, 100Tests×2 | Check More |
PE Anti-Mouse CD366/Tim-3 Antibody[RMT3-23] | 25μg, 100μg | Check More |