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APC Anti-Mouse CD49b/pan-NK cells Antibody[DX5]

Uniprot : Q62469
  • Cat.No.:E-AB-F1116UE

  • Applications: FCM
  • Isotype: Rat IgM, κ
  • Host: Rat

To Purchase E-AB-F1116UE

Size:
  • 25μg
  • 100μg
Price: $65
Qty:

Antigen Details

Alternate Names Integrin alpha-2,CD49 antigen-like family member B,Collagen receptor,Platelet membrane glycoprotein Ia,GPIa,VLA-2 subunit alpha,CD49b
Swissprot Q62469
References
1. Arase H, et al. 2001. J. Immunol. 167:1141.
2. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
3. Sasaki K, et al. 2003. Int. Immunol. 15:701.
4. Inoue O, et al. 2003. J. Cell Biol. 160:769.

Product Details

Form Liquid
Clone No. DX5(See Other Available Formats)
Host Rat
Isotype Rat IgM, κ
Isotype Control APC Rat IgM, κ Isotype Control[RTK2118] [Product E-AB-F09773E]
Reactivity Mouse
Application FCM (See the Application Description)
Storage Buffer Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/106 cells in 100 μL volume].
Shipping Biological ice pack at 4℃
Stability & Storage Keep as concentrated solution.
Store at 2~8°C and protected from prolonged exposure to light. Do not freeze.
Centrifuge before opening to ensure complete recovery of vial contents.
This product is guaranteed up to one year from purchase.
Conjugation APC
APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter).


How to select the appropriate detection channel through the spectrogram?

Background

DX5 antigen has been recently characterized as CD49b. It is a 150 kD integrin α chain also known as α2 integrin, VLA-2 α chain, and integrin α2 chain. CD49b non-covalently associates with CD29 (β1 integrin) to form the CD49b/CD29 complex known as VLA-2, a receptor for collagen and laminin. CD49b is expressed on platelets, the majority of NK cells, NKT cells, and a small subset of CD8+ T cells (this population can be significantly increased following viral infection). DX5 is used for the identification and isolation of NK cells, and is especially useful for identifying NK cells in mice lacking the NK1.1 antigen.

Protocols & Troubleshootings

Related Protocols Staining Cell Surface Targets for Flow Cytometry
Troubleshooting

Troubleshooting for Flow Cytometry

Alternative Formats

Product Name Sizes Price
Biotin Anti-Mouse CD49b/pan-NK cells Antibody[DX5] 25μg, 100μg Check More
FITC Anti-Mouse CD49b/pan-NK cells Antibody[DX5] 50Tests, 100Tests, 100Tests×2 Check More
PE Anti-Mouse CD49b/pan-NK cells Antibody[DX5] 50Tests, 100Tests, 100Tests×2 Check More
APC Anti-Mouse CD49b/pan-NK cells Antibody[DX5] 50Tests, 100Tests, 100Tests×2 Check More
PerCP Anti-Mouse CD49b/pan-NK cells Antibody[DX5] 50Tests, 100Tests, 100Tests×2 Check More
PE/Cyanine5 Anti-Mouse CD49b/pan-NK cells Antibody[DX5] 50Tests, 100Tests, 100Tests×2 Check More
PE/Cyanine7 Anti-Mouse CD49b/pan-NK cells Antibody[DX5] 50Tests, 100Tests, 100Tests×2 Check More
PE/Cyanine5.5 Anti-Mouse CD49b/pan-NK cells Antibody[DX5] 50Tests, 100Tests, 100Tests×2 Check More
PerCP/Cyanine5.5 Anti-Mouse CD49b/pan-NK cells Antibody[DX5] 50Tests, 100Tests, 100Tests×2 Check More
Elab Fluor® 488 Anti-Mouse CD49b/pan-NK cells Antibody[DX5] 50Tests, 100Tests, 100Tests×2 Check More
Elab Fluor® 647 Anti-Mouse CD49b/pan-NK cells Antibody[DX5] 50Tests, 100Tests, 100Tests×2 Check More
Elab Fluor® Violet 450 Anti-Mouse CD49b/pan-NK cells Antibody[DX5] 50Tests, 100Tests, 100Tests×2 Check More
Elab Fluor® Red 780 Anti-Mouse CD49b/pan-NK cells Antibody[DX5] 50Tests, 100Tests, 100Tests×2 Check More
FITC Anti-Mouse CD49b/pan-NK cells Antibody[DX5] 25μg, 100μg Check More
PE Anti-Mouse CD49b/pan-NK cells Antibody[DX5] 25μg, 100μg Check More
PerCP Anti-Mouse CD49b/pan-NK cells Antibody[DX5] 25μg, 100μg Check More
PE/Cyanine5 Anti-Mouse CD49b/pan-NK cells Antibody[DX5] 25μg, 100μg Check More
PE/Cyanine7 Anti-Mouse CD49b/pan-NK cells Antibody[DX5] 25μg, 100μg Check More
PE/Cyanine5.5 Anti-Mouse CD49b/pan-NK cells Antibody[DX5] 25μg, 100μg Check More
PerCP/Cyanine5.5 Anti-Mouse CD49b/pan-NK cells Antibody[DX5] 25μg, 100μg Check More
Elab Fluor® 488 Anti-Mouse CD49b/pan-NK cells Antibody[DX5] 25μg, 100μg Check More
Elab Fluor® 647 Anti-Mouse CD49b/pan-NK cells Antibody[DX5] 25μg, 100μg Check More
Elab Fluor® Violet 450 Anti-Mouse CD49b/pan-NK cells Antibody[DX5] 25μg, 100μg Check More
Elab Fluor® Red 780 Anti-Mouse CD49b/pan-NK cells Antibody[DX5] 25μg, 100μg Check More

Citations

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