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APC Anti-Mouse CD86 Antibody[GL-1]

  • Cat.No.:E-AB-F0994E

  • Applications: FCM
  • Isotype: Rat IgG2a, κ
  • Host: Rat

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Antigen Details

Alternate Names T-lymphocyte activation antigen CD86,Cd86,Activation B7-2 antigen,Early T-cell costimulatory molecule 1,ETC-1
Swissprot P42082
References
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Hathcock KS, et al. 1993. Science 262:905.
3. Freeman GJ, et al. 1993. Science 262:907.
4. Carreno BM, et al. 2002. Annu. Rev. Immunol. 20:29.

Product Details

Form Liquid
Clone No. GL-1(See Other Available Formats)
Host Rat
Isotype Rat IgG2a, κ
Isotype Control APC Rat IgG2a, κ Isotype Control[2A3] [Product E-AB-F09832E]
Reactivity Mouse
Application FCM (See the Application Description)
Storage Buffer Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Shipping Biological ice pack at 4℃
Stability & Storage Keep as concentrated solution.
Store at 2~8°C and protected from prolonged exposure to light. Do not freeze.
Centrifuge before opening to ensure complete recovery of vial contents.
This product is guaranteed up to one year from purchase.
Conjugation APC
APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter).


How to select the appropriate detection channel through the spectrogram?

Background

CD86 is an 80 kD immunoglobulin superfamily member also known as B7-2, B70, and Ly-58. CD86 is expressed on activated B and T cells, macrophages, dendritic cells, and astrocytes. CD86, along with CD80, is a ligand of CD28 and CD152 (CTLA-4). CD86 is expressed earlier in the immune response than CD80. CD86 has also been shown to be involved in immunoglobulin class-switching and triggering of NK cell-mediated cytotoxicity. CD86 binds to CD28 to transduce co-stimulatory signals for T cell activation, proliferation, and cytokine production. CD86 can also bind to CD152, also known as CTLA-4, to deliver an inhibitory signal to T cells.

Protocols & Troubleshootings

Related Protocols Staining Cell Surface Targets for Flow Cytometry
Troubleshooting

Troubleshooting for Flow Cytometry

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

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  1. Advanced Science (2023) IF: 17.521
    Nanomedicines Reprogram Synovial Macrophages by Scavenging Nitric Oxide and Silencing CA9 in Progressive Osteoarthritis

    DOI: 10.1002/advs.202207490

    PMID: 36748885

    Sample: RAW264.7 cell
  2. Frontiers in Immunology (2022) IF: 7.561
    Paeonol Interferes With Quorum-Sensing in Pseudomonas aeruginosa and Modulates Inflammatory Responses In Vitro and In Vivo.

    DOI: 10.3389/fimmu.2022.896874

    Sample: RAW264.7 macrophage cell
  3. Journal of Materials Chemistry B (2022) IF: 7.571
    Fabricating biodegradable calcium phosphate/calcium sulfate cement reinforced with cellulose: in vitro and in vivo studies

    DOI: 10.1039/D2TB02191A

  4. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022) IF: 6.208
    Hepatocyte-Derived Prostaglandin E2-Modulated Macrophage M1-Type Polarization via mTOR-NPC1 Axis-Regulated Cholesterol Transport from Lysosomes to the Endoplasmic Reticulum in Hepatitis B Virus x Protein-Related Nonalcoholic Steatohepatitis

    DOI: 10.3390/ijms231911660

    PMID: 36232960

    Sample: Kupffer cells
  5. Biomaterials Science (2021) IF: 6.843
    Supramolecular copolymer modified statin-loaded discoidal rHDLs for atherosclerotic anti-inflammatory therapy by cholesterol efflux and M2 macrophage polarization

    DOI: 10.1039/D1BM00610J

    PMID: 34346410

    Sample: RAW 264.7 cell
  6. Marine Drugs (2022) IF: 5.118
    In Vitro and In Vivo Dendritic Cell Immune Stimulation Effect of Low Molecular Weight Fucoidan from New Zealand Undaria pinnatifida

    DOI: 10.3390/md20030197

    PMID: 35323496

    Sample: Dendritic cells (DCs)
  7. CERAMICS INTERNATIONAL (2022) IF: 5.532
    Influence of Cu doping on the biocompatibility of zirconia-toughened alumina ceramics for artificial joints

    DOI: 10.1016/j.ceramint.2022.09.291

    Sample: RAW264.7 cell
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