Alternate Names	T-lymphocyte activation antigen CD86,Cd86,Activation B7-2 antigen,Early T-cell costimulatory molecule 1,ETC-1
Swissprot	P42082
References	1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Hathcock KS, et al. 1993. Science 262:905. 3. Freeman GJ, et al. 1993. Science 262:907. 4. Carreno BM, et al. 2002. Annu. Rev. Immunol. 20:29.

Product Details

Form	Liquid
Clone No.	GL-1(See Other Available Formats)
Host	Rat
Isotype	Rat IgG2a, κ
Isotype Control	APC Rat IgG2a, κ Isotype Control[2A3] [Product E-AB-F09832E]
Reactivity	Mouse
Application	FCM (See the Application Description)
Storage Buffer	Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use	Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Shipping	Biological ice pack at 4℃
Stability & Storage	Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Conjugation	APC
	APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter).

How to select the appropriate detection channel through the spectrogram?

Background

CD86 is an 80 kD immunoglobulin superfamily member also known as B7-2, B70, and Ly-58. CD86 is expressed on activated B and T cells, macrophages, dendritic cells, and astrocytes. CD86, along with CD80, is a ligand of CD28 and CD152 (CTLA-4). CD86 is expressed earlier in the immune response than CD80. CD86 has also been shown to be involved in immunoglobulin class-switching and triggering of NK cell-mediated cytotoxicity. CD86 binds to CD28 to transduce co-stimulatory signals for T cell activation, proliferation, and cytokine production. CD86 can also bind to CD152, also known as CTLA-4, to deliver an inhibitory signal to T cells.

Protocols & Troubleshootings

Related Protocols	Staining Cell Surface Targets for Flow Cytometry
Troubleshooting	Troubleshooting for Flow Cytometry

Alternative Formats

Product Name	Sizes	Price
AF/LE Purified Anti-Mouse CD86 Antibody[GL-1]	50μg, 500μg, 1mg	Check More
Purified Anti-Mouse CD86 Antibody[GL-1]	25μg, 100μg	Check More
Biotin Anti-Mouse CD86 Antibody[GL-1]	25μg, 100μg	Check More
FITC Anti-Mouse CD86 Antibody[GL-1]	50Tests, 100Tests, 100Tests×2	Check More
PE Anti-Mouse CD86 Antibody[GL-1]	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine7 Anti-Mouse CD86 Antibody[GL-1]	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5.5 Anti-Mouse CD86 Antibody[GL-1]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 488 Anti-Mouse CD86 Antibody[GL-1]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 647 Anti-Mouse CD86 Antibody[GL-1]	50Tests, 100Tests, 100Tests×2	Check More
PE/Elab Fluor^® 594 Anti-Mouse CD86 Antibody[GL-1]	50Tests, 100Tests, 100Tests×2	Check More
FITC Anti-Mouse CD86 Antibody[GL-1]	25μg, 100μg	Check More
PE Anti-Mouse CD86 Antibody[GL-1]	25μg, 100μg	Check More
APC Anti-Mouse CD86 Antibody[GL-1]	25μg, 100μg	Check More
PE/Cyanine7 Anti-Mouse CD86 Antibody[GL-1]	25μg, 100μg	Check More
PE/Cyanine5.5 Anti-Mouse CD86 Antibody[GL-1]	25μg, 100μg	Check More
Elab Fluor^® 488 Anti-Mouse CD86 Antibody[GL-1]	25μg, 100μg	Check More
Elab Fluor^® 647 Anti-Mouse CD86 Antibody[GL-1]	25μg, 100μg	Check More
PE/Elab Fluor^® 594 Anti-Mouse CD86 Antibody[GL-1]	25μg, 100μg	Check More

Citations

Advanced Science (2023) IF: 17.521
Nanomedicines Reprogram Synovial Macrophages by Scavenging Nitric Oxide and Silencing CA9 in Progressive Osteoarthritis

DOI: 10.1002/advs.202207490

PMID: 36748885
Sample: RAW264.7 cell
Frontiers in Immunology (2022) IF: 7.561
Paeonol Interferes With Quorum-Sensing in Pseudomonas aeruginosa and Modulates Inflammatory Responses In Vitro and In Vivo.

DOI: 10.3389/fimmu.2022.896874

Sample: RAW264.7 macrophage cell
Journal of Materials Chemistry B (2022) IF: 7.571
Fabricating biodegradable calcium phosphate/calcium sulfate cement reinforced with cellulose: in vitro and in vivo studies

DOI: 10.1039/D2TB02191A
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022) IF: 6.208
Hepatocyte-Derived Prostaglandin E2-Modulated Macrophage M1-Type Polarization via mTOR-NPC1 Axis-Regulated Cholesterol Transport from Lysosomes to the Endoplasmic Reticulum in Hepatitis B Virus x Protein-Related Nonalcoholic Steatohepatitis

DOI: 10.3390/ijms231911660

PMID: 36232960
Sample: Kupffer cells
Biomaterials Science (2021) IF: 6.843
Supramolecular copolymer modified statin-loaded discoidal rHDLs for atherosclerotic anti-inflammatory therapy by cholesterol efflux and M2 macrophage polarization

DOI: 10.1039/D1BM00610J

PMID: 34346410
Sample: RAW 264.7 cell
Marine Drugs (2022) IF: 5.118
In Vitro and In Vivo Dendritic Cell Immune Stimulation Effect of Low Molecular Weight Fucoidan from New Zealand Undaria pinnatifida

DOI: 10.3390/md20030197

PMID: 35323496
Sample: Dendritic cells (DCs)
CERAMICS INTERNATIONAL (2022) IF: 5.532
Influence of Cu doping on the biocompatibility of zirconia-toughened alumina ceramics for artificial joints

DOI: 10.1016/j.ceramint.2022.09.291

Sample: RAW264.7 cell

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