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Aspartate Aminotransferase (AST/GOT) Activity Assay Kit

Citations(54)

  • Cat.No.:E-BC-K236-M

  • Detection instrument: Microplate reader(500-520 nm)

Manual MSDS

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  • 96T
  • 48T
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Detection principle

AST/GOT enables alpha-ketoglutaric acid and aspartic acid to displace amino and keto groups to form glutamic acid and oxaloacetic acid. Oxaloacetic acid can decarboxylate itself to form Pyroracemic acid during the reaction. Pyroracemic acid reacted with 2,4-dinitrophenylhydrazine(DNPH) to form 2,4, dinitrophenylhydrazone showing reddish brown in alkaline solution. Measure the OD values and calculate the enzyme activity.

Performance characteristics

Synonyms AST,GOT,Glutamic-oxalacetic transaminase
Sample type Serum,plasma,animal tissue,cells,cell culture supernatant
Sensitivity 1.1 IU/L
Detection range 1.1-72.3 IU/L
Detection method Colorimetric method
Assay type Enzyme Activity
Assay time 80 min
Precision Average inter-assay CV: 6.800%Average intra-assay CV: 5.300%
Other instruments required Micropipettor, Vortex mixer, Incubator, Multichannel pipette
Other reagents required Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage 2-8℃
Valid period 12 months

Images

No significant difference of AST activity was observed between WT and 3SA mice after HFD for 17 weeks.

H Xia et al investigate the relationship between insulin resistance and GSK3β-FBXW7-ERRα transcriptional axis. Aspartate aminotransferase (AST) activity in mouse serum was determined using AST activity assay kit (E-BC-K236-M).

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the formal experiment and the detection range (1.1-72.3 IU/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

Human serum

1

Human plasma

1

Porcine serum

1

Rat serum

1

HC-60 cellular supernatant

1

Calu-3 cellular supernatant

1

10% Rat liver tissue homogenization

15-30

10% Rat lung tissue homogenization

2-8

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

Citations

  1. Advanced Science (2023) IF: 17.521
    Phosphomevalonate Kinase Controls β-Catenin Signaling via the Metabolite 5-Diphosphomevalonate

    DOI: 10.1002/advs.202204909

    PMID: 36808719

    		 Sample: Serum
  2. HEPATOLOGY (2018) IF: 14.079
    MicroRNA 15a/16‐1 suppresses aryl hydrocarbon receptor–dependent interleukin‐22 secretion in CD4+ T cells and contributes to immune‐mediated organ injury

    DOI: 10.1002/hep.29573
    Sample: serum
  3. Nature Communications (2022) IF: 14.919
    Insulin action and resistance are dependent on a GSK3β-FBXW7-ERRα transcriptional axis

    DOI: 10.1038/s41467-022-29722-6

    PMID: 35440636

    		 Sample: serum
  4. Molecular Therapy-Nucleic Acids (2022) IF: 10.183
    Chemically modified in-vitro-transcribed mRNA encoding thrombopoietin stimulates thrombopoiesis in mice

    DOI: 10.1016/j.omtn.2022.08.017

    PMID: 36090760

    		 Sample: plasma
  5. ENVIRONMENTAL SCIENCE & TECHNOLOGY (2022) IF: 9.028
    Lipid Metabolic Disorder Induced by Pyrethroids in Nonalcoholic Fatty Liver Disease of Xenopus laevis

    DOI: 10.1021/acs.est.2c00516
    Sample: plasma
  6. JOURNAL OF HAZARDOUS MATERIALS (2020) IF: 9.038
    The combined adverse effects of cis-bifenthrin and graphene oxide on lipid homeostasis in Xenopus laevis

    DOI: 10.1016/j.jhazmat.2020.124876

    PMID: 33360192
  7. ACS Applied Materials & Interfaces (2021) IF: 9.2289
    Synergistic Multimodal Cancer Therapy Using Glucose Oxidase@CuS Nanocomposites

    DOI: 10.1021/acsami.1c12235

    PMID: 34448397

    		 Sample: plasma
  8. Cell Reports (2022) IF: 9.9949
    Human hepatocyte PNPLA3-148M exacerbates rapid non-alcoholic fatty liver disease development in chimeric mice

    DOI: 10.1016/j.celrep.2022.111321

    PMID: 36103835

    		 Sample: serum
  9. ACS Applied Materials & Interfaces (2019) IF: 8.456
    Multifunctional Graphene-Oxide-Reinforced Dissolvable Polymeric Microneedles for Transdermal Drug Delivery

    DOI: 10.1021/acsami.9b19518
    Sample: blood
  10. CELL PROLIFERATION (2022) IF: 8.7550
    Hypothalamic Hnscr regulates glucose balance by mediating central inflammation and insulin signal

    DOI: 10.1111/cpr.13332
    Sample: serum
  11. Cellular and Molecular Gastroenterology and Hepatology (2022) IF: 8.7970
    DNA 6mA Demethylase ALKBH1 Orchestrates Fatty Acid Metabolism and Suppresses Diet-induced Hepatic Steatosis

    DOI: 10.1016/j.jcmgh.2022.08.011

    PMID: 36058506

    		 Sample: serum
  12. Acta Biomaterialia (2021) IF: 8.9469
    An efficient and safe MUC1-dendritic cell-derived exosome conjugate vaccine elicits potent cellular and humoral immunity and tumor inhibition in vivo

    DOI: 10.1016/j.actbio.2021.10.041

    PMID: 34757230

    		 Sample: serum
  13. Acta Biomaterialia (2021) IF: 8.9469
    A solid lipid coated calcium peroxide nanocarrier enables combined cancer chemo/chemodynamic therapy with O2/H2O2 self-sufficiency

    DOI: 10.1016/j.actbio.2020.12.036
    Sample: Serum,Plasma
  14. Antioxidants (2023) IF: 7.675
    Bone Marrow Mesenchymal Stem-Cell-Derived Exosomes Ameliorate Deoxynivalenol-Induced Mice Liver Damage

    DOI: 10.3390/antiox12030588
    Sample: serum
  15. SCIENCE OF THE TOTAL ENVIRONMENT (2021) IF: 7.963
    Polystyrene microplastics induce hepatotoxicity and disrupt lipid metabolism in the liver organoids

    DOI: 10.1016/j.scitotenv.2021.150328

    PMID: 34571217

    		 Sample: liver organoids (LOs)
  16. SCIENCE OF THE TOTAL ENVIRONMENT (2022) IF: 7.963
    Chronic exposure to polyvinyl chloride microplastics induces liver injury and gut microbiota dysbiosis based on the integration of liver transcriptome profiles and full-length 16S rRNA sequencing data

    DOI: 10.1016/j.scitotenv.2022.155984

    PMID: 35588832

    		 Sample: serum
  17. FOOD AND CHEMICAL TOXICOLOGY (2021) IF: 6.025
    Bacillus amyloliquefaciens B10 can alleviate liver apoptosis and oxidative stress induced by aflatoxin B1

    DOI: 10.1016/j.fct.2021.112124

    PMID: 33727180

    		 Sample: Serum
  18. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022) IF: 6.208
    The Citrus Flavonoid Nobiletin Downregulates Angiopoietin-like Protein 3 (ANGPTL3) Expression and Exhibits Lipid-Modulating Effects in Hepatic Cells and Adult Zebrafish Models

    DOI: 10.3390/ijms232012485

    PMID: 36293338

    		 Sample: blood
  19. BIOMEDICINE & PHARMACOTHERAPY (2022) IF: 6.53
    Activation of α7nAChR preserves intestinal barrier integrity by enhancing the HO-1 / STAT3 signaling to inhibit NF-κB activation in mice

    DOI: 10.1016/j.biopha.2022.112733

    PMID: 36068769

    		 Sample: Serum
  20. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE (2023) IF: 6.633
    Soluble epoxide hydrolase and TRPC3 channels jointly contribute to homocysteine-induced cardiac hypertrophy: Interrelation and regulation by C/EBPβ

    DOI: 10.1016/j.bbadis.2023.166643
    Sample: Serum
  21. LIFE SCIENCES (2022) IF: 5.037
    Aloe emodin 3-O-glucoside inhibits cell growth and migration and induces apoptosis of non-small-cell lung cancer cells via suppressing MEK/ERK and Akt signalling pathways

    DOI: 10.1016/j.lfs.2022.120495

    PMID: 35341826

    		 Sample: serum
  22. MOLECULAR NUTRITION & FOOD RESEARCH (2020) IF: 5.309
    Gymnaster Koraiensis Extract Alleviated Metabolic Syndrome Symptoms and Stimulated UCP1‐Independent Energy Consumption via AMPK Activation in White Adipose Tissue

    DOI: 10.1002/mnfr.202000490
    Sample: Serum
  23. Food Bioscience (2022) IF: 5.318
    Ameliorative effects of monascin from red mold rice on alcoholic liver injury and intestinal microbiota dysbiosis in mice

    DOI: 10.1016/j.fbio.2022.102079
    Sample: serum
  24. Foods (2022) IF: 5.561
    Monascuspiloin from Monascus-Fermented Red Mold Rice Alleviates Alcoholic Liver Injury and Modulates Intestinal Microbiota

    DOI: 10.3390/foods11193048

    PMID: 36230124

    		 Sample: serum
  25. Frontiers in Pharmacology (2022) IF: 5.811
    Uncovering the Pharmacological Mechanisms of Gexia-Zhuyu Formula (GXZY) in Treating Liver Cirrhosis by an Integrative Pharmacology Strategy.

    DOI: 10.3389/fphar.2022.793888
    Sample: serum
  26. Frontiers in Pharmacology (2021) IF: 5.811
    Metformin Alleviates Steatohepatitis in Diet-Induced Obese Mice in a SIRT1-Dependent Way

    DOI: 10.3389/fphar.2021.704112

    PMID: 34483906

    		 Sample: liver
  27. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022) IF: 5.924
    Pyruvate Upregulates Hepatic FGF21 Expression by Activating PDE and Inhibiting cAMP–Epac–CREB Signaling Pathway

    DOI: 10.3390/ijms23105490
    Sample: serum
  28. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2021) IF: 5.924
    Atorvastatin Ester Regulates Lipid Metabolism in Hyperlipidemia Rats via the PPAR-signaling Pathway and HMGCR Expression in the Liver

    DOI: 10.3390/ijms222011107

    PMID: 34681767
  29. American Journal of Translational Research (2021) IF: 4.06
    Personalized nursing improves physical condition and life quality of patients undergoing interventional therapy for liver cancer

    PMID: 35035768

    		 Sample: Blood
  30. Food Bioscience (2022) IF: 4.24
    Fatty acids of Pleurotus florida mushroom: Potential molecules for blood glucose control

    DOI: 10.1016/j.fbio.2022.101558
    Sample: blood
  31. JOURNAL OF ETHNOPHARMACOLOGY (2022) IF: 4.36
    Sub-chronic oral toxicity evaluation of herbo-metallic formulation Arshakuthar rasa in rats

    DOI: 10.1016/j.jep.2022.115306

    PMID: 35443217

    		 Sample: serum
  32. DALTON TRANSACTIONS (2021) IF: 4.39
    A new bis-pyrazolylpyridine ruthenium(III) complex as a potential anticancer drug: in vitro and in vivo activity in murine colon cancer

    DOI: 10.1039/D1DT00185J

    PMID: 33982702

    		 Sample: Serum
  33. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE (2020) IF: 4.486
    Mechanistic insights into the effects of SREBP1c on hepatic stellate cell and liver fibrosis

    DOI: 10.1111/jcmm.15614

    PMID: 32678475

    		 Sample: Serum
  34. AMERICAN JOURNAL OF CHINESE MEDICINE (2021) IF: 4.667
    Identification of Chemical Compounds from Artemisia gmelinii using UPLC-QTOF-MS/MS and their Regulatory Effects on Immune Responses in DSS-Induced Colitis Mice

    DOI: 10.1142/S0192415X21500452

    PMID: 33827384

    		 Sample: serum
  35. Frontiers in Immunology (2019) IF: 4.716
    Gal-3 Deficiency Suppresses Novosphyngobium aromaticivorans Inflammasome Activation and IL-17 Driven Autoimmune Cholangitis in Mice

    DOI: 10.3389/fimmu.2019.01309
    Sample: serum
  36. INTERNATIONAL IMMUNOPHARMACOLOGY (2021) IF: 4.932
    Oridonin regulates the polarized state of Kupffer cells to alleviate nonalcoholic fatty liver disease through ROS-NF-κB

    DOI: 10.1016/j.intimp.2021.108290

    PMID: 34717194

    		 Sample: serum
  37. Scientific Reports (2022) IF: 4.996
    Endothelin receptor B enhances liver injury and pro-inflammatory responses by increasing G-protein-coupled receptor kinase-2 expression in primary biliary cholangitis

    DOI: 10.1038/s41598-022-21816-x
    Sample: serum
  38. Cardiovascular Therapeutics (2022) IF: 3.023
    L-Carnitine Alleviates the Myocardial Infarction and Left Ventricular Remodeling through Bax/Bcl-2 Signal Pathway

    DOI: 10.1155/2022/9615674

    PMID: 35692375

    		 Sample: plasma
  39. Frontiers in Physiology (2019) IF: 3.201
    Dietary Bile Acids Enhance Growth, and Alleviate Hepatic Fibrosis Induced by a High Starch Diet via AKT/FOXO1 and cAMP/AMPK/SREBP1 Pathway in Micropterus salmoides

    DOI: 10.3389/fphys.2019.01430

    PMID: 31824338

    		 Sample: Plasma
  40. International Journal of Environmental Research and Public Health (2022) IF: 3.39
    Benefits on Hematological and Biochemical Parameters of a High-Intensity Interval Training Program for a Half-Marathon in Recreational Middle-Aged Women Runners

    DOI: 10.3390/ijerph19010498

    PMID: 35010758

    		 Sample: plasma
  41. JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY (2022) IF: 3.568
    Myrrhone inhibits the progression of hepatic fibrosis by regulating the abnormal activation of hepatic stellate cells

    DOI: 10.1002/jbt.23177

    PMID: 35983967

    		 Sample: serum
  42. METABOLIC BRAIN DISEASE (2022) IF: 3.584
    Hepatoprotective and neuroprotective effect of taxifolin on hepatic encephalopathy in rats

    DOI: 10.1007/s11011-022-00952-3

    PMID: 35298730

    		 Sample: serum
  43. BMC Complementary and Alternative Medicine (2022) IF: 3.659
    Artemisia argyi extract alleviates inflammation in a DSS-induced colitis mouse model and enhances immunomodulatory effects in lymphoid tissues

    DOI: 10.1186/s12906-022-03536-x

    PMID: 35277165

    		 Sample: serum
  44. Molecular Medicine Reports (2020) IF: 2.1
    Pretreatment with the ALDH2 activator Alda‑1 protects rat livers from ischemia/reperfusion injury by inducing autophagy

    DOI: 10.3892/mmr.2020.11312

    PMID: 32705206

    		 Sample: Serum
  45. Evidence-based Complementary and Alternative Medicine (2022) IF: 2.65
    San-Huang-Chai-Zhu Formula Ameliorates Liver Injury in Intrahepatic Cholestasis through Suppressing SIRT1/PGC-1α-Regulated Mitochondrial Oxidative Stress

    DOI: 10.1155/2022/7832540
    Sample: hepatic tissue,serum
  46. JOURNAL OF SEPARATION SCIENCE (2020) IF: 2.878
    High‐throughput metabolomics method based on liquid chromatography‐mass spectrometry: insights into the underlying mechanisms of salinity‐alkalinity exposures‐induced metabolites changes in Barbus capito

    DOI: 10.1002/jssc.202000861
    Sample: Serum
  47. PeerJ (2022) IF: 2.984
    3,4-dihydroxyphenylethyl alcohol glycoside reduces acetaminophen-induced acute liver failure in mice by inhibiting hepatocyte ferroptosis and pyroptosis

    DOI: 10.7717/peerj.13082

    PMID: 35310165

    		 Sample: plasma
  48. Open Life Sciences (2022) IF: 1.311
    β-Hydroxybutyrate upregulates FGF21 expression through inhibition of histone deacetylases in hepatocytes

    DOI: 10.1515/biol-2022-0095
    Sample: serum
  49. Journal of Oleo Science (2022) IF: 1.628
    Chemoprotective Effect of Decalactone on Hepatic Cancer via Diminishing the Inflammatory Response and Oxidative Stress

    DOI: 10.5650/jos.ess22033

    PMID: 35965085

    		 Sample: liver
  50. TOXICOLOGY AND INDUSTRIAL HEALTH (2019) IF: 1.635
    Toxic effects of subchronic oral acetamiprid exposure in rats:

    DOI: 10.1177/0748233719893203
    Sample: plasma
  51. Applied Biological Chemistry (2021) IF: 1.813
    Supplementation of non-fermented and fermented goji berry (Lycium barbarum) improves hepatic function and corresponding lipid metabolism via their anti-inflammatory and antioxidant properties in high fat-fed rats

    DOI: 10.1186/s13765-021-00642-1
    Sample: serum
  52. ACS Applied Bio Materials (2020)
    Noninvasive Visualization of Obesity-Boosted Inflammation in Orthotopic Pancreatic Ductal Adenocarcinoma Using an Octapod Iron Oxide Nanoparticle

    DOI: 10.1021/acsabm.0c00841
    Sample: Serum
  53. Toxicology Reports (2022)
    Effect of silymarin on blood coagulation profile and osmotic fragility in carbon tetrachloride induced hepatotoxicity in male Wistar rats

    DOI: 10.1016/j.toxrep.2022.06.005
    Sample: plasma
  54. Toxicology Reports (2017)
    Diclofenac – induced hepatotoxicity: Low dose of omega-3 fatty acids have more protective effects

    DOI: 10.1016/j.toxrep.2017.12.002
    Sample: plasma
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Reviews/Q&A

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  • Reviews
  • Q&A

Verified Customer

a*****hSubmitted [ Jun 05 2019 ]

  • Application:Biochemical
  • Description:the biggest advantage of this kit is the small sample size.on the other hand,no special drawing tools are required,we can draw a standard curve in excel,which is convenient.
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Q

M*****aSubmitted [ Oct 20 2022 ]

Asked: Is it possible to perform this AST activity assay on frozen samples. For example if I have done the sample preparation on mouse livers, if I store the extracted samples at -80°C for a few nights can I reuse those same samples for the enzyme activity test?

Reply

A

adminSubmitted [ Oct 20 2022 ]

Answered: If you has no time to test on the same day, the tissue can be aliquoted and frozen at -80 degrees. If the supernatant after homogenization is processed, it is not recommended to test overnight, which will affect the enzyme activity. The tissue homogenization can be performed when you needs to test.

Q

M*****aSubmitted [ Sep 20 2022 ]

Asked: I want to know what is the exact difference between the sample and the control reactions. Both wells have all the same reagents added to them and also both have the sample. The sample is just added at different times, does this cause a change in the reaction?

Reply

A

adminSubmitted [ Sep 20 2022 ]

Answered: Set the control is to exclude the interference of pyruvate and non-enzymatic reaction of the sample. Reagent 4 has an inhibitory effect on the enzyme and can terminate the reaction between substrate and enzyme.

Q

s************fSubmitted [ Apr 01 2022 ]

Asked: What is meant by 98T or 48T

Reply

A

adminSubmitted [ Apr 01 2022 ]

Answered: Hi sumaira, Thank you for your effort, we will reply to your email soon.

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