Aspartate Aminotransferase (AST/GOT) Activity Assay Kit (Reitman-Frankel Method)

    • Biochemical Assay Kit-Elabscience
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    • Biochemical Assay Kit-Elabscience
    • Biochemical Assay Kit-Elabscience

      Catalog number:E-BC-K236-M

      Size:
      • 96T
      • 48T
      Qty:
      - +
      Price: $180
      Can detect 43 samples if without duplication

      Detection method: Colorimetric method

      Detection instrument: Microplate reader(500-520 nm)

      Lead Time: 7~10 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request Manual

      Detection principle

      AST/GOT enables alpha-ketoglutaric acid and aspartic acid to displace amino and keto groups to form glutamic acid and oxaloacetic acid. Oxaloacetic acid can decarboxylate itself to form Pyroracemic acid during the reaction. Pyroracemic acid reacted with 2,4-dinitrophenylhydrazine(DNPH) to form 2,4, dinitrophenylhydrazone showing reddish brown in alkaline solution. Measure the OD values and calculate the enzyme activity.

      Performance characteristics

      Synonyms AST,GOT,Glutamic-oxalacetic transaminase
      Sample type serum,plasma,animal tissue,cells,cell culture supernatant
      Sensitivity 1.1 IU/L
      Detection range 1.1-72.3 IU/L
      Precision Average inter-assay CV: 6.8%Average intra-assay CV: 5.3%
      Other instruments required Micropipettor, Vortex mixer, Incubator, Multichannel pipette
      Other reagents required Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
      Storage 2-8℃
      Valid period 6 months

      Citations

      1. Liver ischemia/reperfusion injury, a setting in which the functional mass is reduced and the role of PDE5 inhibitor
        IF: 2.638
        Journal: Eur. J. Anat(2019)
        Products: E-BC-K235-M 
        Sample type: Serum
        Reactivity: Rat
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      Verified Customer

      a*****hSubmitted [ Jun 05 2019 ]

      • Application:Biochemical
      • Description:the biggest advantage of this kit is the small sample size.on the other hand,no special drawing tools are required,we can draw a standard curve in excel,which is convenient.
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