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Aspartate Aminotransferase (AST/GOT) Activity Fluorometric Assay Kit

Cat.No.:E-BC-F043
Detection instrument: Fluorescence microplate reader (Ex/Em=535 nm/587 nm)

Detection method: Fluorescence method

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48T

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Detection principle

AST can catalyze the substrate to produce pyruvate. Pyruvate is oxidized to produce hydrogen peroxide, which causes the fluorescent probe to produce fluoresce. The activity of AST can be calculated by measuring the increase of fluorescence value at the excitation wavelength of 535 nm and the emission wavelength of 587 nm.

Performance characteristics

Synonyms	AST、GOT
Sample type	cell, animal tissue,serum (plasma) and other liquid samples
Sensitivity	0.03 U/L
Detection range	0.03-1.5 U/L
Detection method	Fluorescence method
Assay type	Enzyme Activity
Assay time	80 min
Precision	Average inter-assay CV: 7.300%Average intra-assay CV: 2.200%
Other instruments required	Micropipette, Vortex mixer, Centrifuge
Storage	-20℃
Valid period	12 months

Ralated product

Cat Number	Product Name
E-BC-F038	Alanine Aminotransferase (ALT/GPT) Activity Fluorometric Assay Kit

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Collection and treatment of common samples for metabolism assay detection

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