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Aspartate Aminotransferase (AST/GOT) Activity Fluorometric Assay Kit

  • Cat.No.:E-BC-F043

  • Detection instrument: Fluorescence microplate reader (Ex/Em=535 nm/587 nm)

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Price: $400

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Detection principle

AST can catalyze the substrate to produce pyruvate. Pyruvate is oxidized to produce hydrogen peroxide, which causes the fluorescent probe to produce fluoresce. The activity of AST can be calculated by measuring the increase of fluorescence value at the excitation wavelength of 535 nm and the emission wavelength of 587 nm.

Performance characteristics

Synonyms AST、GOT
Sample type cell, animal tissue,serum (plasma) and other liquid samples
Sensitivity 0.03 U/L
Detection range 0.03-1.5 U/L
Detection method Fluorescence method
Assay type Enzyme Activity
Assay time 80 min
Precision Average inter-assay CV: 7.300%Average intra-assay CV: 2.200%
Other instruments required Micropipette, Vortex mixer, Centrifuge
Storage -20℃
Valid period 12 months



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