Alanine Aminotransferase (ALT/GPT) Activity Fluorometric Assay Kit (E-BC-F038)
For research use only.
| Detection Principle | ALT catalyze the amino conversion reaction between alanine and α-ketoglutaric acid to produce pyruvic acid and glutamic acid. Under the action of pyruvate oxidase, pyruvic acid generates H2O2, which reacts with the non-fluorescent substance to form fluorescent substance under the action of peroxidase. The activity of ALT can be calculated by measuring the increase of fluorescence value at the excitation wavelength of 535 nm and the emission wavelength of 590 nm. |
| Synonyms | ALT |
| Sample Type | Serum,Plasma,Animal tissue |
| Detection Method | Fluorometric method |
| Detection Instrument | Fluorescence microplate reader (Ex/Em=535 nm/587 nm) |
| Research Area | Liver And Renal Function |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.01 U/L |
| Detection Range | 0.01-0.83 U/L |
| Precision | inter-assay CV: 9.8% | intra-assay CV: 2.3% |
| Sample Volume | 20 μL |
| Assay Time | 1 h 15 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 10-15 |
| Dog serum | 5-10 |
| Rat serum | 10-15 |
| 10% Mouse heart tissue homogenate | 100-120 |
| 10% Rat spleen tissue homogenate | 10-15 |
| 10% Rat liver tissue homogenate | 300-500 |
| 10% Rat kidney tissue homogenate | 100-120 |
| 10% Rat lung tissue homogenate | 100-120 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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