Aspartate Aminotransferase (AST/GOT) Activity Fluorometric Assay Kit (E-BC-F043)
For research use only.
| Detection Principle | Aspartate aminotransferase (AST/GOT) is an important indicator of liver inflammation. When cells are damaged, the permeability of cell membrane increases, resulting in the release of AST in the cytoplasm into the blood and the increase of serum AST activity. AST can catalyze the substrate to produce pyruvate. Pyruvate is oxidized to produce hydrogen peroxide, which causes the fluorescent probe to produce fluoresce. The activity of AST can be calculated by measuring the increase of fluorescence value at the excitation wavelength of 535 nm and the emission wavelength of 587 nm. |
| Synonyms | AST;GOT |
| Sample Type | Cell,Animal tissue,Serum,Plasma,Other liquid sample |
| Detection Method | Fluorometric method |
| Detection Instrument | Fluorescence microplate reader (Ex/Em=535 nm/587 nm) |
| Research Area | Liver And Renal Function |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.03 U/L |
| Detection Range | 0.03-1.5 U/L |
| Precision | inter-assay CV: 7.3% | intra-assay CV: 2.2% |
| Sample Volume | 20 μL |
| Assay Time | 1 h 20 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Rat heart tissue homogenate | 300-600 |
| 10% Rat brain tissue homogenate | 300-500 |
| 10% Rat liver tissue homogenate | 200-500 |
| Human serum | 5-15 |
| 10% Rat kidney tissue homogenate | 200-500 |
| Rat serum | 10-20 |
| 10% Rat spleen tissue homogenate | 50-100 |
| 293T cell(1 × 10^6) | 10-20 |
| 10% Mouse lung tissue homogenate | 100-200 |
| 293T cell supernatant | 5-15 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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