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Biuret Protein Colorimetric Assay Kit

  • Cat.No.:E-BC-K165-M

  • Detection instrument: Microplate reader(520-580 nm,optimum wavelength: 540 nm)

To Purchase E-BC-K165-M

Size:
  • 96T
  • 500Assays
Price: $100
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Detection principle

Any compound that contains two -CONH2 in the molecule can react with alkaline copper solution to form a purple complex, which is known as the biuret reaction. Many peptide bonds (-CONH-) in protein molecules can perform this reaction, and the color degree of all kinds of proteins are essentially the same.

Performance characteristics

Synonyms TP
Sample type Serum,plasma,tissue
Sensitivity 0.58 g/L
Detection range 0.58-100 g/L
Detection method Colorimetric method
Assay type Quantitative
Assay time 20 min
Precision Average inter-assay CV: 6.5%Average intra-assay CV: 4%
Other instruments required Micropipettor, Incubator, Vortex mixer, Centrifuge
Other reagents required Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage Reagent 3: -20℃, others: 2-8℃
Valid period 6 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.58-100 g/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

Human serum

1

Human plasma

1

Rat serum

2-4

Mouse plasma

1

Rabbit serum

1

Chicken plasma

1

Horse serum

1-3

Porcine serum

1-3

Dog serum

2-4

10% Rat spleen tissue homogenate

1

10% Mouse liver tissue homogenate

1

10% Mouse kidney tissue homogenate

1

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).


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