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Biuret Protein Colorimetric Assay Kit

Cat.No.:E-BC-K165-M
Detection instrument: Microplate reader(520-580 nm,optimum wavelength: 540 nm)

Detection method: Colorimetric method

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96T
500Assays

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Detection principle

Any compound that contains two -CONH2 in the molecule can react with alkaline copper solution to form a purple complex, which is known as the biuret reaction. Many peptide bonds (-CONH-) in protein molecules can perform this reaction, and the color degree of all kinds of proteins are essentially the same.

Performance characteristics

Synonyms	TP
Sample type	Serum,plasma,tissue
Sensitivity	0.58 g/L
Detection range	0.58-100 g/L
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	20 min
Precision	Average inter-assay CV: 6.500%Average intra-assay CV: 4%
Other instruments required	Micropipettor, Incubator, Vortex mixer, Centrifuge
Other reagents required	Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage	Reagent 3: -20℃, others: 2-8℃
Valid period	12 months

Images

TP level was significantly induced in HFD/STZ treated group.

A A-R Mohamed et al found that selenium nanoparticle can alleviate cardio-hepatic damage in type II diabetes. Total protein (TP) level in rat serum was determined using biuret protein colorimetric assay kit (E-BC-K165-M).

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.58-100 g/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Human serum	1
Human plasma	1
Rat serum	2-4
Mouse plasma	1
Rabbit serum	1
Chicken plasma	1
Horse serum	1-3
Porcine serum	1-3
Dog serum	2-4
10% Rat spleen tissue homogenate	1
10% Mouse liver tissue homogenate	1
10% Mouse kidney tissue homogenate	1

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

Journal of Oleo Science (2022) IF: 1.628
Chemoprotective Effect of Decalactone on Hepatic Cancer via Diminishing the Inflammatory Response and Oxidative Stress

DOI: 10.5650/jos.ess22033

PMID: 35965085
Sample: serum

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