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Bradford Protein Colorimetric Assay Kit

Cat.No.:E-BC-K168-M
Detection instrument: Microplate reader(550 nm-630 nm, optimum wavelength: 595 nm)

Detection method: Colorimetric method

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96T
500Assays

Price: $100

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Protocols for metabolism assays

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Detection principle

Coomassie brilliant blue G-250 is red under the free state, and it has the maximum absorbance at 465 nm. When the Coomassie brilliant blue G-250 combined to protein, the compound will have the maximum at 595 nm. The absorbance value is directly proportional to the protein content, so the concentration of total protein can be calculated directly by measuring the OD value at 595 nm.

Performance characteristics

Synonyms	TP
Sample type	Serum,plasma,animal tissue and cell
Sensitivity	0.046 mg/mL
Detection range	0.046-0.6 mg/mL
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	20 min
Precision	Average inter-assay CV: 8.200%Average intra-assay CV: 3.200%
Other instruments required	Micropipettor, Incubator, Vortex mixer, Centrifuge
Other reagents required	Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage	Reagent 2: RT, Reagent 1: 2-8℃
Valid period	12 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range ( MERGEFIELD Detection_range 0.046-0.6 mg/mL).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Human serum	90-110
Human plasma	90-110
Rabbit serum	90-110
Rat plasma	90-110
Chicken serum	90-110
10% Mouse kidney tissue homogenate	15-20
10% Mouse lung tissue homogenate	15-20
10% Rat spleen tissue homogenate	15-20
10% Rat heart tissue homogenate	15-20
10% Rat liver tissue homogenate	20-25
293T	1
HL-60	1

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

Journal of Oleo Science (2022) IF: 1.628
Chemoprotective Effect of Decalactone on Hepatic Cancer via Diminishing the Inflammatory Response and Oxidative Stress

DOI: 10.5650/jos.ess22033

PMID: 35965085
Sample: serum

Reviews/Q&A

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Reviews
Q&A

M*****aSubmitted [ Aug 31 2022 ]

Asked: Can the plate in this kit be replaced with our own 96-well plate?

adminSubmitted [ Aug 31 2022 ]

Answered: Yes, it can be replaced with the same type of microplate.

M*****aSubmitted [ Aug 17 2022 ]

Asked: Can the kit work on plant sample?

adminSubmitted [ Aug 17 2022 ]

Answered: No, it can not detect the plant sample, But there is E-BC-K318-M which is suitable for plant tissue.

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