Current Position:Home>>Kits>>Metabolism Assays>> Bradford Protein Colorimetric Assay Kit

Bradford Protein Colorimetric Assay Kit

Cat.No.:E-BC-K168-S
Detection instrument: Spectrophotometer(595 nm)

Detection method: Colorimetric method

To Purchase E-BC-K168-S

Size:

100Assays
500Assays

Price: $100

Qty:

Welcome to order from local distributors.

Protocols for metabolism assays

FAQ for metabolism assays

Have Questions?

Add to cart Bulk request

For research use only. Order now, ship in 3 days

Detection principle

Coomassie brilliant blue G-250 is red under the free state, and it has the maximum absorbance at 465 nm. When the Coomassie brilliant blue G-250 combined to protein, the compound will have the maximum at 595 nm. The absorbance value is directly proportional to the protein content, so the concentration of total protein can be calculated directly by measuring the OD value at 595 nm.

Performance characteristics

Synonyms	TP
Sample type	Serum,plasma,animal tissue
Sensitivity	0.026 mg/mL
Detection range	0.026-1.2 mg/mL
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	50 min
Precision	Average inter-assay CV: 2.300%Average intra-assay CV: 1.800%
Other instruments required	Micropipettor, Vortex mixer
Other reagents required	Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage	Reagent 1: 2-8℃, Reagent 2: RT
Valid period	12 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.026-1.2 mg/mL).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Human serum	40-60
10% Mouse liver tissue homogenization	8-12
Rat serum	40-60

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

Microorganisms (2020) IF: 4.152
Visual Detection of Clostridium perfringens Alpha Toxin by Combining Nanometer Microspheres with Smart Phones

DOI: 10.3390/microorganisms8121865

PMID: 33256026
Journal of Oleo Science (2022) IF: 1.628
Chemoprotective Effect of Decalactone on Hepatic Cancer via Diminishing the Inflammatory Response and Oxidative Stress

DOI: 10.5650/jos.ess22033

PMID: 35965085
Sample: serum

Reviews/Q&A

Show all
Reviews
Q&A

D****aSubmitted [ Nov 06 2023 ]

Asked: Can i use cell lysate in microplate reader for this kit?

adminSubmitted [ Nov 06 2023 ]

Answered: 1. E-BC-K168-S kit can detect serum, plasma and animal tissue samples. E-BC-K168-M is recommended if you want to test cell samples. 2. Our kit is based on the principle of chemical reaction, and the components in the lysate are very complex, which may have an impact on the kit reaction system, so it's not recommended to treat cell samples with lysate. It is recommended that cell samples be broken by mechanical homogenization or ultrasonic homogenization.

M*****aSubmitted [ Oct 10 2022 ]

Asked: Is Bradford the same as Coomassie Brilliant blue method?

adminSubmitted [ Oct 10 2022 ]

Answered: Bradford is another statement of Coomassie Brilliant Blue. It's the same thing.

M*****aSubmitted [ Sep 27 2022 ]

Asked: Is Bradford the same as Coomassie Brilliant blue method?

adminSubmitted [ Sep 27 2022 ]

Answered: Bradford is another statement of Coomassie Brilliant Blue. It's the same thing.

M*****aSubmitted [ Sep 14 2022 ]

Asked: How long is the shelf life of this kit? What is the standard for this kit?

adminSubmitted [ Sep 14 2022 ]

Answered: The shelf life of this kit is 6 months and the standard is BSA.

... Show All Show Less

Cat. No	E-BC-K168-S
Application
Sample Type

*Product Name:
*Catalog Number:
*Name:
*Email:
*Message:
*Country:
*When will you use it?	Within 1 month In 2 to 3 month In 4 to 6 month 6 months later
*Captcha:	Click to load Captcha