Bradford Protein Colorimetric Assay Kit

    • Biochemical Assay Kit-Elabscience
    • Biochemical Assay Kit-Elabscience
    • Biochemical Assay Kit-Elabscience

      Catalog number:E-BC-K168-S

      • 100Assays
      • 500Assays
      - +
      Price: $100
      Can detect 96 samples if without duplication

      Detection method: Colorimetric method

      Detection instrument: Spectrophotometer(595 nm)

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      Detection principle

      Coomassie brilliant blue G-250 is red under the free state, and it has the maximum absorbance at 465 nm. When the Coomassie brilliant blue G-250 combined to protein, the compound will have the maximum at 595 nm. The absorbance value is directly proportional to the protein content, so the concentration of total protein can be calculated directly by measuring the OD value at 595 nm.

      Performance characteristics

      Synonyms TP
      Sample type Serum,plasma,animal tissue
      Sensitivity 0.026 mg/mL
      Detection range 0.026-1.2 mg/mL
      Detection method Colorimetric method
      Assay type Quantitative
      Assay time 50 min
      Precision Average inter-assay CV: 2.3%Average intra-assay CV: 1.8%
      Other instruments required Micropipettor, Vortex mixer
      Other reagents required Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
      Storage Reagent 1: 2-8℃, Reagent 2: RT
      Valid period 6 months
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      MSDS for ELISA ISO 9001

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