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Bradford Protein Colorimetric Assay Kit

  • Cat.No.:E-BC-K168-S

  • Detection instrument: Spectrophotometer(595 nm)

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Detection principle

Coomassie brilliant blue G-250 is red under the free state, and it has the maximum absorbance at 465 nm. When the Coomassie brilliant blue G-250 combined to protein, the compound will have the maximum at 595 nm. The absorbance value is directly proportional to the protein content, so the concentration of total protein can be calculated directly by measuring the OD value at 595 nm.

Performance characteristics

Synonyms TP
Sample type Serum,plasma,animal tissue
Sensitivity 0.026 mg/mL
Detection range 0.026-1.2 mg/mL
Detection method Colorimetric method
Assay type Quantitative
Assay time 50 min
Precision Average inter-assay CV: 2.300%Average intra-assay CV: 1.800%
Other instruments required Micropipettor, Vortex mixer
Other reagents required Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage Reagent 1: 2-8℃, Reagent 2: RT
Valid period 12 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.026-1.2 mg/mL).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

Human serum

40-60

10% Mouse liver tissue homogenization

8-12

Rat serum

40-60

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Microorganisms (2020) IF: 4.152
    Visual Detection of Clostridium perfringens Alpha Toxin by Combining Nanometer Microspheres with Smart Phones

    DOI: 10.3390/microorganisms8121865

    PMID: 33256026

  2. Journal of Oleo Science (2022) IF: 1.628
    Chemoprotective Effect of Decalactone on Hepatic Cancer via Diminishing the Inflammatory Response and Oxidative Stress

    DOI: 10.5650/jos.ess22033

    PMID: 35965085

    Sample: serum

Reviews/Q&A

  • Show all
  • Reviews
  • Q&A
Q

D****aSubmitted [ Nov 06 2023 ]

Asked: Can i use cell lysate in microplate reader for this kit?

Reply

A

adminSubmitted [ Nov 06 2023 ]

Answered: 1. E-BC-K168-S kit can detect serum, plasma and animal tissue samples. E-BC-K168-M is recommended if you want to test cell samples. 2. Our kit is based on the principle of chemical reaction, and the components in the lysate are very complex, which may have an impact on the kit reaction system, so it's not recommended to treat cell samples with lysate. It is recommended that cell samples be broken by mechanical homogenization or ultrasonic homogenization.

Q

M*****aSubmitted [ Oct 10 2022 ]

Asked: Is Bradford the same as Coomassie Brilliant blue method?

Reply

A

adminSubmitted [ Oct 10 2022 ]

Answered: Bradford is another statement of Coomassie Brilliant Blue. It's the same thing.

Q

M*****aSubmitted [ Sep 27 2022 ]

Asked: Is Bradford the same as Coomassie Brilliant blue method?

Reply

A

adminSubmitted [ Sep 27 2022 ]

Answered: Bradford is another statement of Coomassie Brilliant Blue. It's the same thing.

Q

M*****aSubmitted [ Sep 14 2022 ]

Asked: How long is the shelf life of this kit? What is the standard for this kit?

Reply

A

adminSubmitted [ Sep 14 2022 ]

Answered: The shelf life of this kit is 6 months and the standard is BSA.

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