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Calcium (Ca) Colorimetric Assay Kit

Cat.No.:E-BC-K103-M
Detection instrument: Microplate reader(600-620 nm,optimum wavelength: 610 nm)

Detection method: Colorimetric method

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Protocols for metabolism assays

FAQ for metabolism assays

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Detection principle

Calcium ion in the sample bind to Methyl Thymol Blue (MTB) in alkaline solution and form blue complex. The blue complex has a specific absorption peak at 715nm and calcium content can be calculated by measuring the OD value at 610 nm.

Performance characteristics

Synonyms	Ca
Sample type	Serum,plasma,urine,tissue
Sensitivity	0.07 mmol/L
Detection range	0.07-1.2 mmol/L
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	20 min
Precision	Average inter-assay CV: 8.500%Average intra-assay CV: 4.700%
Other instruments required	Micropipettor, Centrifuge, Incubator, Vortex mixer
Storage	2-8℃
Valid period	12 months

Images

The levels of Ca concentration was significantly reduced in PD5 group. (**P<0.01)

TH Huang et al developed a surface decorated peptide nanoparticle that can be used for osteoporosis and diabetes therapy. Calcium (Ca) level in rat plasma was determined using Ca colorimetric assay kit (E-BC-K103-M).

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.07-1.2 mmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Dog serum	2-3
Human serum	3-6
Mouse serum	3-6
Human urine	4-8
20% Animal tissue homogenate	1

Note: The diluent is deionized water.

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

ADVANCED FUNCTIONAL MATERIALS (2022) IF: 19.924
Surface Decoration of Peptide Nanoparticles Enables Efficient Therapy toward Osteoporosis and Diabetes

DOI: 10.1002/adfm.202210627

Sample: plasma
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2022) IF: 8.025
Impact of Lycium barbarum polysaccharide on the expression of glucagon-like peptide 1 in vitro and in vivo

DOI: 10.1016/j.ijbiomac.2022.10.176

PMID: 36283558
Sample: STC1 cells
BIOCHEMICAL PHARMACOLOGY (2022) IF: 6.1
Pterostilbene-isothiocyanate impedes RANK/TRAF6 interaction to inhibit osteoclastogenesis, promoting osteogenesis in vitro and alleviating glucocorticoid induced osteoporosis in rats

DOI: 10.1016/j.bcp.2022.115284

PMID: 36209841
Sample: serum
JOURNAL OF NUTRITIONAL BIOCHEMISTRY (2022) IF: 6.117
Vitamin D protects silica particles induced lung injury by promoting macrophage polarization in a KLF4-STAT6 manner

DOI: 10.1016/j.jnutbio.2022.109148

PMID: 36049670
Sample: serum
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2021) IF: 6.291
Upregulation of autophagy in M2 macrophage by vitamin D alleviates crystalline silica-induced pulmonary inflammatory damage

DOI: 10.1016/j.ecoenv.2021.112730

PMID: 34478973
Sample: lung
Frontiers in Plant Science (2022) IF: 6.627
Halotolerant Bacillus altitudinis WR10 improves salt tolerance in wheat via a multi-level mechanism

DOI: 10.3389/fpls.2022.941388

PMID: 35909740
Sample: roots or shoots
LIFE SCIENCES (2022) IF: 6.78
Empagliflozin enhances neuroplasticity in rotenone-induced parkinsonism: Role of BDNF, CREB and Npas4

DOI: 10.1016/j.lfs.2022.121258

Sample: brain
INTERNATIONAL IMMUNOPHARMACOLOGY (2023) IF: 5.714
HC067047 as a potent TRPV4 inhibitor repairs endotoxemia colonic injury

DOI: 10.1016/j.intimp.2022.109648

Sample: colon
Frontiers in Physiology (2023) IF: 4.755
Relative comparison of chronic kidney disease-mineral and bone disorder rat models

DOI: 10.3389/fphys.2023.1083725

Sample: Serum
Frontiers in Oncology (2020) IF: 4.848
The Pan-Sirtuin Inhibitor MC2494 Regulates Mitochondrial Function in a Leukemia Cell Line

DOI: 10.3389/fonc.2020.00820

PMID: 32528892
Sample: Cell lysate
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY (2020) IF: 3.292
Chronic exposure of bisphenol A impairs carbohydrate and lipid metabolism by altering corresponding enzymatic and metabolic pathways

DOI: 10.1016/j.etap.2020.103387

PMID: 32339907
Sample: Serum
JOURNAL OF INVERTEBRATE PATHOLOGY (2020) IF: 2.074
Potential Muscle Activity Disturbance in Penaeus monodon during Acute Hepatopancreatic Necrosis Disease (AHPND) Infection: Inference through Gene Expression, Calcium Concentration, and MicroRNA

DOI: 10.1016/j.jip.2020.107497

PMID: 33130047
Sample: Tissue homogenate
Animals (2021) IF: 2.752
Date Palm Pollen Extract Avert Doxorubicin-Induced Cardiomyopathy Fibrosis and Associated Oxidative/Nitrosative Stress, Inflammatory Cascade, and Apoptosis-Targeting Bax/Bcl-2 and Caspase-3 Signaling Pathways

DOI: 10.3390/ani11030886

PMID: 33804672
Sample: Serum

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Reviews/Q&A

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Reviews
Q&A

R******PSubmitted [ Dec 12 2023 ]

Asked: What is the dilution factor for Cell culture supernatants?

adminSubmitted [ Dec 12 2023 ]

Answered: Hello Ramesh,our laboratory verified the Ca concentration in HepG2 cell supernatant, diluted by 2 times and measured to 1.340 mmol/L. However, there are differences between different samples, it is recommended that you choose 2-3 samples for pre-experiment to determine the dilution factor.

M***********gSubmitted [ Jul 24 2023 ]

Asked: May I ask if I can analyse calcium content using cell lysate samples? I didn\'t see this sample being included in the protocol so I would like to ask further.

adminSubmitted [ Jul 24 2023 ]

Answered: Hi Sir, I'll reply to your mail

M*****iSubmitted [ Oct 27 2022 ]

Asked: What anticoagulant should be used for plasma sample?

adminSubmitted [ Oct 27 2022 ]

Answered: It is recommended to use heparin as an anticoagulant, do not use calcium chelating agent and oxalate.

M*****iSubmitted [ Sep 20 2022 ]

Asked: The reagent 3 is kind of solid. What should I do to use it?

adminSubmitted [ Sep 20 2022 ]

Answered: Preheat the reagent 3 at 37℃ until clarified before use.

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