Calcium (Ca) Colorimetric Assay Kit (E-BC-K103-M)
For research use only.
| Detection Principle | Calcium ion in the sample bind to Methyl Thymol Blue (MTB) in alkaline solution and form blue complex. The blue complex has a specific absorption peak at 715 nm and calcium content can be calculated by measuring the OD value at 610 nm. |
| Synonyms | Ca |
| Sample Type | Serum,Plasma,Urine,Tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (600-620 nm, optimum wavelength: 610 nm) |
| Research Area | Plant Physiology Series , Inorganic Ions |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.07 mmol/L |
| Detection Range | 0.07-1.2 mmol/L |
| Precision | inter-assay CV: 8.5% | intra-assay CV: 4.7% |
| Sample Volume | 10 μL |
| Assay Time | 20 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Dog serum | 2-3 |
| Human serum | 3-6 |
| Mouse serum | 3-6 |
| Human urine | 4-8 |
| 20% Animal tissue homogenate | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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