Caspase 8, also known as FLICE, MACH and Mch5 is self-activated or activated in response to granzyme B cleavage. Caspase 8 specifically recognizes IETD. Activated caspase 8 can not only cleave PARP, but also participate in the activation of other ICE family proteases, such as caspase 3 and caspase 7. This caspase 8 activity assay kit is based on caspase 8 that can catalyze the substrate Ac-IETD-pNA to generate yellow pNA (p-Nitroaniline). The pNA has a strong absorption near 405 nm, The activity of caspase 8 can be calculated by determining the absorbance at 405 nm.

General Information

Application	Caspase
Assay type	Qualitative or quantitative
Detection method	Colorimetric Method
Sample type	Cell and tissue samples
Assay time	4-6 hours
Detection instrument	Spectrophotometer
Other reagents required	PBS(E-BC-R187)，Bradford Protein Determination Kit(E-BC-K168-S or E-BC-K168-M)
Storage	-20°C, shading light
Shipping	Ice bag
Expiration date	12 months

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