Catalase (CAT) Activity Assay Kit

    • Biochemical Assay Kit-Elabscience
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    • Biochemical Assay Kit-Elabscience
    • Biochemical Assay Kit-Elabscience

      Catalog number:E-BC-K031-M

      Size:
      • 96T
      • 48T
      Qty:
      - +
      Price: $150
      Can detect 40 samples if without duplication

      Detection method: Colorimetric method

      Detection instrument: Microplate reader(400-410 nm,optimum wavelength: 405 nm)

      Lead Time: Order now, ship in 3 daysWelcome to order from local distributors.

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      Detection principle

      The reaction that catalase (CAT) decomposes H2O2 can be quickly stopped by ammonium molybdate. The residual H2O2 reacts with ammonium molybdate to generate a yellowish complex. CAT activity can be calculated by production of the yellowish complex at 405 nm.

      Performance characteristics

      Synonyms CAT
      Sample type Serum,plasma,cells,cell culture supernatant,tissue
      Sensitivity 1.12 U/mL
      Detection range 1.12 -150 U/mL
      Detection method Colorimetric method
      Assay type Enzyme Activity
      Assay time 40 min
      Precision Average inter-assay CV: 7.7%Average intra-assay CV: 3.9%
      Other instruments required Micropipettor, Vortex Mixer, Incubator
      Other reagents required Normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4)
      Storage 2-8℃
      Valid period 6 months

      Dilution of sample

      It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (1.12 -150 U/mL).

      The recommended dilution factor for different samples is as follows (for reference only):

      Sample type

      Dilution factor

      Human serum

      1

      293T supernatant

      1

      10% Rat heart tissue homogenization

      50-100

      10% Rat liver tissue homogenization

      100-200

      10% Rat spleen tissue homogenization

      50-100

      Mouse serum

      1

      10% Epipremnum aureum tissue homogenization

      1-2

      10% Rat lung tissue homogenization

      50-100

      10% Rat kidney tissue homogenization

      50-100

      10% Rat brain tissue homogenization

      20-50

      Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).


      Citations

      1. Quercetin supplemented casein-based extender improves the post-thaw quality of rooster semen
        IF: 2.283
        Journal: Cryobiology(2020)
        DOI: 10.1016/j.cryobiol.2020.04.010
        PMID: 32437678
        Products: E-BC-K022-M E-BC-K031-M E-BC-K096-M 
        Sample type: Sperm
        Reactivity: Chicken
      2. The Hepatoprotective Effect of Moringa Oleifera Leaf Aqueous Extract Against Cadmium-Induced Toxicity on Male Rats
        Journal: Journal of Biochemical Technology(2020)
        DOI: 99999999
        PMID: 99999999
        Products: E-BC-K022-M E-BC-K025-M E-BC-K030-M E-BC-K031-M 
        Sample type: Serum,Tissue homogenate
        Reactivity: Rat
      3. Hepatoprotective and antioxidant activities of Pterocarpus santalinoides methanol leaf extract
        Journal: African Journal of Pharmacy and Pharmacology(2019)
        DOI: 10.5897/AJPP2019.5090
        PMID: 99999999
        Products: E-BC-K020-M E-BC-K031-M 
        Sample type: Serum
        Reactivity: Rat
      4. Histopathological and Biochemical Biomarker Response of Mussel, Unio Pictorum, to Carbamate Pesticide Carbaryl: A Laboratory Study
        IF: 0.395
        Journal: Indian Journal of Animal Research(2019)
        DOI: 10.18805/ijar.B-1157
        PMID: 99999999
        Products: E-BC-K020-M E-BC-K025-M E-BC-K030-M E-BC-K031-M E-BC-K096-M 
        Sample type: Tissue homogenate
        Reactivity: Mussel
      5. Omega-3 fatty acids moderate oxidative and proinflammatory events in experimental hepatotoxicity in Wistar rats: comparison with livolin
        Journal: Synergy(2018)
        DOI: 10.1016/j.synres.2018.08.001
        PMID: 99999999
        Products: E-BC-K025-M E-BC-K031-M E-BC-K091-M E-BC-K096-M E-BC-K210 E-BC-K235-M E-BC-K236-M E-EL-0076 
        Sample type: Plasma
        Reactivity: Rat
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      Verified Customer

      m***ySubmitted [ May 31 2019 ]

      • Application:Biochemical
      • Description:I used this Catalase (CAT) kit to test rat liver homogenate. the detection process require a small sample size and takes a little time .
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      MSDS for ELISA ISO 9001

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