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Catalase (CAT) Activity Fluorometric Assay Kit

Cat:E-BC-F006 Citations (1)
Manual MSDS

Price: $ 300

Size:
96T
Quantity:
  • Sample Type: serum;plasma; animal tissue
  • Detection Instrument: Fluorescence microplate reader (Ex/Em=535 nm/587 nm)
  • Reasearch Areas: Oxidative stress;Plant Physiology Series
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For research use only. Order now, ship in 3 days

Product Details
Detection Principle Catalase can decompose H2O2 to generate H2O and O2, the residual H2O2 in the detection system react with the fluorescent substance, and the content of residual H2O2 is proportional to the fluorescence intensity at the excitation wavelength of 535 nm and emission wavelength of 587 nm, the catalase activity is inversely proportional to the fluorescence intensity.
Synonyms CAT
Sample Type serum,plasma, animal tissue
Sensitivity 0.01 U/L
Detection Range 0.01-6.51 U/L
Detection Method Fluorescence method
Assay Time 30 min
Precision inter-assay CV: 6.8% | intra-assay CV: 3.4%
Storage This product can be stored at -20°C for 12 months with shading light.
Valid Period 12 months
Dilution Of Sample

The recommended dilution factor for different samples is as follows (for reference only):

Sample type Dilution factor
Mouse serum 60-80
Mouse plasma 60-80
Rat serum 70-80
Human saliva 30-50
Rat urine 30-50
10% Mouse liver tissue homogenate 2500-3000
10% Mouse lung tissue homogenate 200-400
10% Rat muscle tissue homogenate 100-200
10% Rat brain tissue homogenate 40-50
10% Mouse kidney tissue homogenate 2000-2500
The diluent is buffer solution. For the dilution of other sample types, please do pretest to confirm the dilution factor.