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Cell Ferrous Iron Colorimetric Assay Kit

Cat.No.:E-BC-K881-M
Detection instrument: Microplate reader (590-600 nm, optimum wavelength: 593 nm）

Detection method: Colorimetric method

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96T
48T

Price: $300

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Performance characteristics

Sample type	cells
Sensitivity	0.4 μmol/L
Detection range	0.4-50 μmol/L
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	40 min
Precision	Average inter-assay CV: 1.500%Average intra-assay CV: 1.300%
Storage	2-8℃
Valid period	12 months

Images

Intracellular ferrous iron was significantly increased in cells treated with TNF-α. (***P<0.0001)

Y Zhang et al investigate the ferroptosis of renal tubular epithelial cells that promoting chronic renal allograft interstitial fibrosis. Cell ferrous ion level in HK-2 cell were determined using cell ferrous ion colorimetric assay kit (E-BC-K881-M).

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

FREE RADICAL BIOLOGY AND MEDICINE (2022) IF: 8.1010
IRF1/ZNF350/GPX4-mediated ferroptosis of renal tubular epithelial cells promote chronic renal allograft interstitial fibrosis

DOI: 10.1016/j.freeradbiomed.2022.11.002

Sample: HK-2 cell
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2023) IF: 6.208
HBV Enhances Sorafenib Resistance in Hepatocellular Carcinoma by Reducing Ferroptosis via SRSF2-Mediated Abnormal PCLAF Splicing

DOI: 10.3390/ijms24043263

Sample: HepG2 cell
ENVIRONMENTAL TOXICOLOGY (2023) IF: 4.109
GPX4 utilization by selenium is required to alleviate cadmium-induced ferroptosis and pyroptosis in sheep kidney

DOI: 10.1002/tox.23740

Sample: Kidney
JOURNAL OF APPLIED TOXICOLOGY (2023) IF: 3.628
Hydrogen sulfide alleviates beryllium sulfate-induced ferroptosis and ferritinophagy in 16HBE cells

DOI: 10.1002/jat.4453

Sample: 16HBE cell

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